Purpose: This report describes a case of Descemet membrane endothelial keratoplasty (DMEK) graft failure after non-clearing bullae over the area of the orientation mark on the graft.
Methods: Case report.
Results: The summary of the clinical presentation and workup is described, followed by a brief overview of the DMEK procedure and the gentian violet (GV) ink used to ensure the correct orientation of the DMEK graft.
Conclusions: GV has a good safety profile; however, there are rare cases of adverse events. Therefore, alternative approaches should be explored, such as the use of intraoperative optical coherence tomography, reviewing a video recording of the insertion step, adjusting the insertion technique, or using asymmetrical trephine marking on graft edges.
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http://dx.doi.org/10.1016/j.ajoc.2024.102056 | DOI Listing |
Biosensors (Basel)
November 2024
Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon 16419, Republic of Korea.
Crystal violet (CV) is a disinfectant and antifungal agent used in aquaculture that plays a vital role in treating aquatic diseases and sterilizing water. However, its potential for strong toxicity, including carcinogenicity and mutagenicity, upon accumulation in the body raises concerns regarding its safe use. Therefore, there is a growing need for the quantitative detection of CV in its early application stages to ensure human safety.
View Article and Find Full Text PDFMethods Mol Biol
December 2024
Department of Veterinary Pathobiology, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX, USA.
Modified Vaccinia virus Ankara (MVA) is a promising vaccine vector with an outstanding safety profile. The gold standard method to titrate MVA is to immune stain plaques formed in MVA-infected monolayer of primary chicken embryo fibroblasts. Recently, DF-1, an immortal chicken embryo fibroblast line, has also been used.
View Article and Find Full Text PDFJ Nepal Health Res Counc
October 2024
Department of ENT-HNS, National Academy of Medical Sciences, Kathmandu, Nepal.
Background: Otomycosis is a fungal infection of external auditory canal with worldwide prevalence of 9% to 30%. Treatment includes elimination of predisposing factor, thorough canal cleaning and topical or systemic antifungal agents. Clotrimazole is a specific topical antifungal agent with effectiveness varying from 95% to 100%.
View Article and Find Full Text PDFIndian J Otolaryngol Head Neck Surg
December 2024
Department Of Otorhinolaryngology, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam, Tamil Nadu 603103 India.
Chronic suppurative otitis media is a prolonged and chronic condition. It is a prevalent bacterial infection in the field of otolaryngology that has substantial consequences. Prior to surgical intervention, it is crucial to implement medical treatment to make the ears dry in cases of chronic suppurative otitis media.
View Article and Find Full Text PDFInt J Biol Macromol
December 2024
National & Local Joint Engineering Research Center for Mineral Salt Deep Utilization, Huaiyin Institute of Technology, Huai'an, 223003, China; Department of Applied and Basic Sciences, School of Applied Health Sciences, A'Sharqiyah University, P.O. Box 42, Ibra 400, Sultanate of Oman. Electronic address:
The primary concern is the large amounts of emitted organic dyes through wastewater from numerous sectors. One of the most hazardous dyes commonly used in the industrial sector is methyl violet. It has been known to cause skin, gastric, and respiratory disorders.
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