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Disentangling hydroxynitrile glucoside biosynthesis in a barley (Hordeum vulgare) metabolon provides access to elite malting barleys for ethyl carbamate-free whisky production. | LitMetric

AI Article Synopsis

  • - Barley produces several specialized metabolites called hydroxynitrile glucosides (HNGs), with α-HNG epiheterodendrin being problematic in whisky production due to its role in forming harmful ethyl carbamate during distillation.
  • - Research utilized metabolite-genome-wide association studies (GWAS) to identify genetic traits and candidate genes related to the levels of HNGs, discovering wild barley lines with significant differences in their HNG ratios.
  • - The study explored the roles of specific genes in HNG biosynthesis, revealing that changes in these genes don't affect resistance to a certain fungal pathogen, while also identifying a gene line that could aid in breeding barley with reduced ethyl carbamate risk.

Article Abstract

Barley produces several specialized metabolites, including five α-, β-, and γ-hydroxynitrile glucosides (HNGs). In malting barley, presence of the α-HNG epiheterodendrin gives rise to undesired formation of ethyl carbamate in the beverage production, especially after distilling. Metabolite-GWAS identified QTLs and underlying gene candidates possibly involved in the control of the relative and absolute content of HNGs, including an undescribed MATE transporter. By screening 325 genetically diverse barley accessions, we discovered three H. vulgare ssp. spontaneum (wild barley) lines with drastic changes in the relative ratios of the five HNGs. Knock-out (KO)-lines, isolated from the barley FIND-IT resource and each lacking one of the functional HNG biosynthetic genes (CYP79A12, CYP71C103, CYP71C113, CYP71U5, UGT85F22 and UGT85F23) showed unprecedented changes in HNG ratios enabling assignment of specific and mutually dependent catalytic functions to the biosynthetic enzymes involved. The highly similar relative ratios between the five HNGs found across wild and domesticated barley accessions indicate assembly of the HNG biosynthetic enzymes in a metabolon, the functional output of which was reconfigured in the absence of a single protein component. The absence or altered ratios of the five HNGs in the KO-lines did not change susceptibility to the fungal phytopathogen Pyrenophora teres causing net blotch. The study provides a deeper understanding of the organization of HNG biosynthesis in barley and identifies a novel, single gene HNG-0 line in an elite spring barley background for direct use in breeding of malting barley, eliminating HNGs as a source of ethyl carbamate formation in whisky production.

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Source
http://dx.doi.org/10.1111/tpj.16768DOI Listing

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