gen. nov., sp. nov. of fam. nov. and sp. nov. from forest soil and proposal of fam. nov.

Two Gram-negative, aerobic, rod-shaped bacterial strains, 7MK25 and 6Y81, were isolated from forest soil of Dinghushan Biosphere Reserve, Guangdong Province, PR China. Based on the results of 16S rRNA gene sequence analysis, strain 7MK25 showed the highest similarity (93.6 %) to AR4, followed by DSM 9653 (93.3 %). Strain 6Y81 had the highest similarity of 97.9 % to RmlP026, followed by RmlP001 (97.2 %). Phylogenomic analysis using the UBCG and PhyloPhlAn methods consistently showed that strain 7MK25 formed a sister clade to , while strain 6Y81 formed an independent clade within the genus , both in the order . The digital DNA-DNA hybridization and average nucleotide identity values between strains 7MK25, 6Y81 and their close relatives were in the ranges of 19.1-29.9 % and 72.5-85.5 %, respectively. The major fatty acids of 7MK25 were summed feature 8 (C ω7/C ω6), C cyclo ω8, C and C cyclo, while those of 6Y81 were summed feature 8 (C ω7/C ω6), C and C 3-OH. Strains 7MK25 and 6Y81 took diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine as their dominant polar lipids, and Q-10 as their major respiratory quinone. On the basis of phenotypic and phylogenetic data, strain 7MK25 is proposed to represent a novel species of a novel genus with name gen. nov., sp. nov., within a novel family fam. nov., with 7MK25 (=KCTC 62738=GDMCC 1.1452) as its type strain. Strain 6Y81 represents a novel species in the genus , for which the name sp. nov. (type strain 6Y81=KACC 21 727=GDMCC 1.2176) is proposed. fam. nov. with as the type genus is also proposed to solve the non-monophylectic problem of the family .