Green Synthesis of Molybdenum Nanoparticles From Solanum xanthocarpum and Evaluation of Their Antimicrobial and Antioxidant Activity Against Multidrug-Resistant Wound Isolates.

Introduction: In recent years, antimicrobial drug resistance has emerged as a serious global public health concern, according to the World Health Organization data. The emergence of pathogens resistant to multiple drugs has been linked to an increase in morbidity and mortality from microbial infections. The study's main goal is to explore the efficacy of using in the green synthesis of molybdenum nanoparticles (Mo NPs) for antibacterial and antioxidant properties.

Methods: An eco-friendly method of synthesizing Mo NPs was accomplished using an aqueous extract of . Characterization of the synthesized nanoparticles was done by UV-visible spectroscopy (UV-Vis), Fourier-transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), scanning electron microscope (SEM), and energy-dispersive X-ray spectroscopy (EDX). After that, antibacterial and antioxidant activity was further evaluated.

Results: The UV-visible spectrophotometer analysis confirmed the presence of synthesized Mo NPs showing a peak around 320 nm. The presence of functional compounds like C-CI, C-H, C=C, and O=C=O was confirmed by FT-IR spectrum analysis. The positions of diffraction peaks in Mo NP patterns were identified using XRD analysis; they were more crystalline (82.7%) and less amorphous (17.3%). The presence of the elements molybdenum (Mo), carbon (C), and oxygen (O) was confirmed by the EDX spectrum and irregular shapes shown in the SEM images. Further, the antimicrobial study results showed the formation of an inhibition zone against 27 mm for , 24 mm for , 22 mm for , and 24 mm for , respectively, at a high concentration 80 μg/ml of Mo NPs. The maximum antioxidant activity at 100 μg/ml was 73.49%, compared to the standard ascorbic acid (74.25%). Additionally, the moderate activity at 60 μg/ml was 53.21%, compared to the standard (56.5%), and the minimal activity at 20 μg/ml was 30.21%, compared to the standard (36.89%).

Conclusion: The environmentally friendly synthesized Mo NPs from exhibited antioxidant activity. Furthermore, the findings show that Mo NPs mediated by can inhibit antibiotic-resistant bacteria, especially methicillin-resistant , , , and . In order to understand further how nanoparticles work against bacteria that are resistant to many drugs, additional research and clinical studies would be needed.