Compounds isolation from leaf extract against the species in shrimp through bioassay-guided fractionation.

This study was conducted to isolate and identify the bioactive compounds from the ethanolic extract of leaf against species through a bioassay-guided fractionation. The ethanol extract was exposed to silica gel chromatography followed by reversed phase HPLC to isolate the most effective fraction against . Using further UHPLC-orbitrap-ion trap mass spectrometry, five compounds were isolated with broad-spectrum potency against a range of Vibrio species viz. and The IC values for the compounds ranged from 8 to 48 µg/mL against the most sensitive species and 58 to >400 µg/mL against The results of the toxicity tests demonstrated that the compounds were not harmful for shrimp. The study's findings indicate that leaf extract may contain bioactive molecules that are able to be substituted for antibiotics to treat vibriosis in shrimp farming.