isolated from giant panda can attenuate dextran sodium sulfate-induced colitis in mice.

Objective: Probiotics are beneficial to the intestinal barrier, but few studies have investigated probiotics from giant pandas. This study aims to explore the preventive effects of giant panda-derived on dextran sodium sulfate (DSS)-induced colitis in mice.

Methods: was administered to mice 14 days before administering DSS treatment to induce enteritis.

Results: B14 could more effectively prevent colitis in mice than B13. B14 protected the mouse colon by decreasing the histology index and serum interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) levels, which improved intestinal inflammation-related symptoms. In addition, the treatment led to the regulation of the expression of and , which in turn inhibited immune pathways. The expression of , , , , and is related the intestinal mucosal barrier. 16S sequencing shows that the B14 significantly increased the abundance of certain intestinal probiotics. Overall, B14 exerted a preventive effect on colitis in mice by inhibiting immune responses, enhancing the intestinal barrier and increasing the abundance of probiotic species. Thus, B14 administration helps regulate the balance of the intestinal microecology. It can suppress immune pathways and enhance barrier-protective proteins.