AI Article Synopsis
- Long non-coding RNAs (lncRNAs) show promise as predictive markers for gastric cancer, and the natural compound coumarin may enhance this effect.
- The study investigated how coumarin impacted three specific lncRNAs (BANCR, MALAT1, and FER1L4) and their target genes (PTEN, p-PI3K, and p-AKT) in AGS gastric cancer cells using various assays.
- Results indicated that coumarin decreased cell viability, reduced expression of target mRNAs linked to BANCR, and led to lower protein levels of PTEN, p-PI3K, and p-AKT, suggesting coumarin could be a viable treatment option for gastric cancer.
Article Abstract
Because long non-coding RNAs (lncRNAs) can affect several interconnected processes, its value as a predictive marker for gastric cancer has been demonstrated. Coumarin - a natural compound known to contain some beneficial antitumor qualities - was tested for its effects on AGS gastric cancer cells. In this study, we investigated the expression level of selected cellular lncRNAs (BANCR, MALAT1 and FER1L4) and their target genes (PTEN, p-PI3K and p-AKT) in coumarin-treated AGS cell line. The expressions of the three lncRNAs: BANCR, MALAT1 and FER1L4, as well as their specified targets, PTEN, PI3K and AKT, were measured by qRT-PCR. To gauge the impact of coumarin on the AGS cells, a MTT assay was utilized. A Western blot has been employed to assess variations in PTEN, p-PI3K, and p-AKT expression. The experiment's results showed that AGS viability diminished with increasing doses of coumarin. Compared to the control cells, the cells exposed to coumarin had showed reduced levels of mRNAs which are known targets of the lncRNA BANCR. At the same time, levels of lncRNAs MALAT1 and FER1L4 within coumarin group have been higher comparing to those within control group. Additionally, the Western blot analysis revealed that the coumarin-treated cells expressed lower levels of p-PI3K, PTEN as well as p-AKT compared to control group. This information points to coumarin being a possible option in a treatment regimen for gastric cancer due to its ability to affect lncRNAs and the molecules they target.
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- Long non-coding RNAs (lncRNAs) show promise as predictive markers for gastric cancer, and the natural compound coumarin may enhance this effect.
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