AI Article Synopsis
- Microbial fermentation can be done using two main strategies: one where growth is linked to production (growth-coupled) and another where they are separate (nongrowth-coupled).
- Utilizing stoichiometric metabolic models with flux balance analysis helps enhance the engineering processes for target synthesis, particularly in growth-coupled systems, which can also aid in overcoming production bottlenecks through evolution.
- For cost-effective production of bulk chemicals, adopting a nongrowth-coupled approach is essential; this requires careful management of the transition from growth to production modes and ensuring cellular activity remains high even when not growing.
Article Abstract
Microbial fermentation employs two strategies: growth- and nongrowth-coupled productions. Stoichiometric metabolic models with flux balance analysis enable pathway engineering to couple target synthesis with growth, yielding numerous successful results. Growth-coupled engineering also contributes to improving bottleneck flux through subsequent adaptive evolution. However, because growth-coupled production inevitably shares resources between biomass and target syntheses, the cost-effective production of bulk chemicals mandates a nongrowth-coupled approach. In such processes, understanding how and when to transition the metabolic state from growth to production modes becomes crucial, as does maintaining cellular activity during the nongrowing state to achieve high productivity. In this paper, we review recent technologies for growth-coupled and nongrowth-coupled production, considering their advantages and disadvantages.
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| http://dx.doi.org/10.1016/j.copbio.2024.103133 | DOI Listing |
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