Protocol for evaluating mutualistic cooperative behavior in mice using a water-reward task assay.

STAR Protoc

Jiangsu Key Laboratory of Neurodegeneration, Nanjing Medical University, Nanjing 211166, China; Center for Global Health, Nanjing Medical University, Nanjing 211166, China; Brain Institute, Nanjing Brain Hospital, Nanjing Medical University, Nanjing 210029, China; Changzhou Medical Center, Nanjing Medical University, Changzhou 213003, China. Electronic address:

Published: June 2024

AI Article Synopsis

  • - The study explores social cooperation in mice, which is crucial for group behaviors but often overlooked due to their aggressive nature.
  • - A new water-reward assay was developed to study mutualistic cooperative behavior in mice, detailing the setup and methodology for researchers.
  • - This validated protocol allows investigators to analyze and quantify cooperation in mice, with references provided for further details on the execution.

Article Abstract

Social cooperation is fundamentally important for group animals but rarely studied in mice because of their natural aggressiveness. Here, we present a new water-reward assay to investigate mutualistic cooperative behavior in mice. We describe the construction of the apparatus and provide details of the procedures and analysis for investigators to characterize and quantify the mutualistic cooperative behavior. This protocol has been validated in mice and can be used for investigating mechanisms of cooperation. For complete details on the use and execution of this protocol, please refer to Zhang et al. and Wang et al..

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11047788PMC
http://dx.doi.org/10.1016/j.xpro.2024.103023DOI Listing

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