(MTB) is known for its adaptive capability in developing resistance to antibiotics, through the selection of spontaneous mutations that arise during treatment. Generating spontaneous antibiotic-resistant mutants is challenging but necessary for studying this phenomenon. A protocol was designed and tested to select stable, MTB spontaneous, d-cycloserine (DCS) resistant mutants. Twenty-four colonies resistant to DCS were selected, demonstrating an increase between 1 and 4 times the Minimum Inhibitory Concentration (MIC) set for H37Rv ATCC 27294 reference strain.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11024651 | PMC |
| http://dx.doi.org/10.1016/j.mex.2024.102690 | DOI Listing |
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