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A novel colorimetric assay for the detection of urinary N, N-diacetylspermine, a known biomarker for colorectal cancer.
Anal Biochem
February 2025
Department of Biological Sciences, University of Alberta, Edmonton, AB T6G 2E9, Canada; Department of Computer Sciences, University of Alberta, Edmonton, AB, T6G 2E8, Canada; Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, T6G 1C9, Canada; Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB, T6G 2H7, Canada. Electronic address:
Article Synopsis
- Urinary N, N-diacetylspermine (DAS) is a low-concentration biomarker for colorectal cancer, but current detection methods are complex and costly, creating a demand for simpler alternatives, especially for low-resource settings.
- This study introduces an efficient, inexpensive colorimetric assay using recombinant diacetylspermine oxidase (rDAS Ox) to oxidize DAS, which produces hydrogen peroxide and generates a pink color proportional to DAS levels in urine.
- A novel two-column ion exchange resin protocol was developed to remove interfering metabolites and concentrate DAS, enabling detection of as little as 1 μM DAS and demonstrating effective correlation with known DAS concentrations in urine samples.
Comprehensive evaluation of the nephrotoxicity of carbon quantum dots: Effects of the surface charge.
Chemosphere
November 2024
Department of Civil Engineering, National Taipei University of Technology, Taipei City, 106, Taiwan; Department of Biology, University of Michigan-Flint, MI, 48502, USA. Electronic address:
Article Synopsis
- Carbon quantum dots (CQDs) are being studied for their biomedical applications, and this research assesses their safety and health risks based on different surface modifications.
- The study examines three types of CQDs, highlighting variations in their physicochemical properties and biological impacts, particularly cytotoxicity, oxidative stress, and inflammation response.
- Findings suggest that while some CQDs can induce cytotoxic effects and oxidative stress, they don't significantly harm renal integrity or cause organ damage in vivo, indicating their potential for safe use in biomedicine.
The Spermine Oxidase/Spermine Axis Coordinates ATG5-Mediated Autophagy to Orchestrate Renal Senescence and Fibrosis.
Adv Sci (Weinh)
August 2024
Department of Nephrology, The First Affiliated Hospital, Sun Yat-sen University, NHC Key Laboratory of Clinical Nephrology, Guangdong Provincial Key Laboratory of Nephrology, Guangzhou, Guangdong, 510080, China.
Decreased plasma spermine levels are associated with kidney dysfunction. However, the role of spermine in kidney disease remains largely unknown. Herein, it is demonstrated that spermine oxidase (SMOX), a key enzyme governing polyamine metabolism, is predominantly induced in tubular epithelium of human and mouse fibrotic kidneys, alongside a reduction in renal spermine content in mice.
View Article and Find Full Text PDFIdentifying Predictive Biomarkers of Subclinical Mastitis in Dairy Cows through Urinary Metabotyping.
Metabolites
April 2024
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, Canada.
Article Synopsis
- * This study investigated metabolic changes in cows at risk for subclinical mastitis (SCM) by analyzing urine samples collected 8 and 4 weeks before calving, identifying 27 metabolites that differentiate SCM-prone cows from healthy ones.
- * Four specific metabolites showed predictive power for identifying SCM risk, suggesting that metabolite testing could facilitate early interventions to improve dairy cow management and reduce SCM impacts, although further validation is necessary.
Spermidine from arginine metabolism activates Nrf2 and inhibits kidney fibrosis.
Commun Biol
June 2023
Department of Medicine and Clinical Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
Kidney metabolism may be greatly altered in chronic kidney disease. Here we report that arginine metabolism is the most altered in unilateral ureteral obstruction (UUO)-induced fibrosis of the kidneys in metabolomic analysis. Spermidine is the most increased metabolite of arginine.
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