Cloning and functional identification of apple LATERAL ORGAN BOUNDARY DOMAIN 3 (LBD3) transcription factor in the regulation of drought and salt stress.

Planta

Apple Technology Innovation Center of Shandong Province, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, National Key Laboratory of Wheat Improvement, College of Horticulture Science and Engineering, Shandong Agricultural University, Taian, 271018, Shandong, China.

Published: April 2024

AI Article Synopsis

  • Overexpression of the MdLBD3 gene in Arabidopsis enhances the plant's resistance to salt and drought stress while also promoting early flowering.
  • The study identifies and analyzes the LATERAL ORGAN BOUNDARY DOMAIN (LBD) protein family in apples, noting the limited understanding of their roles in woody plants.
  • Findings suggest that MdLBD3, localized in the nucleus and expressed in various tissues, could be a valuable genetic resource for breeding apples with improved tolerance to environmental stresses.

Article Abstract

Overexpression of MdLBD3 in Arabidopsis reduced sensitivity to salt and drought stresses and was instrumental in promoting early flowering. Salt and drought stresses have serious effects on plant growth. LATERAL ORGAN BOUNDARY DOMAIN (LBD) proteins are a plant-specific transcription factors (TFs) family and play important roles in plants in resisting to abiotic stress. However, about the function of LBDs in apple and other woody plants is little known. In this study, protein sequences of the LBD family TFs in apples were identified which contained conserved LOB domains. The qRT-PCR analysis showed that the MdLBD3 gene was widely expressed in various tissues and organs. The subcellular localization assay showed that the MdLBD3 protein was localized in the nucleus. Ectopic expression of MdLBD3 in Arabidopsis positively regulated its salt and drought resistance, and promoted early flowering. Collectively, these results showed that MdLBD3 improved the abiotic stress resistance, plant growth and development. Overall, this study provided a new gene for breeding that can increase the abiotic stress tolerance in apple.

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Source
http://dx.doi.org/10.1007/s00425-024-04373-7DOI Listing

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