Fat deposition affects beef quantity and quality via preadipocyte proliferation. Beta-sitosterol, a natural small molecular compound, has various functions, such as anti-inflammation, antibacterial, and anticancer properties. The mechanism of action of Beta-sitosterol on bovine preadipocytes remains unclear. This study, based on RNA-seq, reveals the impact of Beta -sitosterol on the proliferation of bovine preadipocytes. Compared to the control group, Beta-sitosterol demonstrated a more pronounced inhibitory effect on cell proliferation after 48 hours of treatment than after 24 hours, as evidenced by the results of EdU staining and flow cytometry. RNA-seq and Western Blot analyses further substantiated these findings. Our results suggest that the impact of Beta-sitosterol on the proliferation of bovine preadipocytes is not significant after a 24-hour treatment. It is only after extending the treatment time to 48 hours that Beta-sitosterol may induce cell cycle arrest at the G2/M phase by suppressing the expression of CCNB1, thereby inhibiting the proliferation of bovine preadipocytes.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1080/10495398.2024.2339406 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Association of R3HDM1 variants with growth and meat quality traits in Qinchuan cattle and its role in lipid accumulation.
Gene
December 2024
College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China; National Beef Cattle Improvement Centre, Yangling 712100, China. Electronic address:
The R3H domain containing 1 (R3HDM1) gene has emerged as a candidate influencing residual feed intake and beef yield. Despite this, the genetic variation of R3HDM1 and its effects on beef cattle remain unexplored. This study identified four single nucleotide polymorphisms (SNPs) in the R3HDM1 gene of Qinchuan cattle, with the g.
View Article and Find Full Text PDFBta-miR-484 regulates proliferation and apoptosis of bovine intramuscular preadipocytes via targeting MAP3K9 to inhibit the JNK signaling pathway.
Int J Biol Macromol
November 2024
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, PR China; National Beef Cattle Improvement Center, Northwest A&F University, Yangling, Shaanxi 712100, PR China. Electronic address:
Intramuscular fat (IMF) plays a crucial role in enhancing the tenderness, flavor, and juiciness of beef, making the increase of IMF content a significant objective in beef breeding. A key factor influencing IMF levels is the number of intramuscular preadipocytes. Previous studies have indicated a correlation between bta-miR-484 and IMF content.
View Article and Find Full Text PDFLncBNIP3 Inhibits Bovine Intramuscular Preadipocyte Differentiation via the PI3K-Akt and PPAR Signaling Pathways.
J Agric Food Chem
November 2024
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China.
Intramuscular fat (IMF) content is an economic trait in beef cattle that improves the meat quality. Studies have highlighted the correlation between long noncoding RNAs (lncRNAs) and IMF development. In this study, lncBNIP3 knockdown promoted bovine intramuscular preadipocyte differentiation.
View Article and Find Full Text PDFsnRNA-seq of adipose tissues reveals the potential cellular and molecular mechanisms of cold and disease resistance in Mongolian cattle.
BMC Genomics
October 2024
Grassland & Cattle Investment Co., Ltd, Hohhot, 011500, China.
Background: Mongolian cattle are local breeds in northern China with excellent adaptability to harsh environmental conditions. Adipose tissues play essential roles in tolerance to cold and disease, but the associated cellular and molecular mechanisms are unclear.
Methods: Single-nucleus RNA sequencing (snRNA-seq) was performed on the adipose tissues from the subcutaneous (SAT), greater omentum (OAT) and perirenal (PAT) of 3 healthy cattle.
Combined Multi-Omics Analysis Reveals the Potential Role of in Yak Intramuscular Fat Deposition.
Int J Mol Sci
August 2024
Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Sichuan Province and Ministry of Education, Southwest Minzu University, Chengdu 610225, China.
Article Synopsis
- - The study focuses on yaks, a unique livestock breed from the Qinghai-Tibet Plateau, emphasizing the importance of intramuscular fat (IMF) for beef quality.
- - Researchers used RNA-Seq and Protein-Seq techniques to analyze the tissue of yaks with varying IMF levels, identifying five key genes related to fat content.
- - Results indicate that one specific gene inhibits the differentiation and proliferation of yak intramuscular preadipocytes, affecting fat storage by modulating the PPARα signaling pathway, which could enhance our understanding of yak meat quality.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!