AI Article Synopsis
- Reconstructed epidermis models, derived from 3D keratinocyte cultures, are important for testing skin safety and efficacy, highlighting the need for thorough evaluation of their barrier function.
- This study uses speckle analysis and Raman microspectroscopy on Reconstructed Human Epidermis (RHE) samples at different maturation stages to assess the skin barrier.
- Results showed significant improvements in key parameters between maturation days D17 and D20, confirming that the speckle technique is an effective method for evaluating skin barrier properties in these models.
Article Abstract
Background: Reconstructed epidermis models, obtained from 3D keratinocytes culture, have gained significant prominence as prototypes for safety and efficacy testing in skin research. To effectively evaluate these models, it is essential to perform molecular and functional characterization. The skin's barrier function is one of the essential aspects of the epidermis that needs to be assessed. A noninvasive method is thus required for the evaluation of the skin barrier in these models. With this perspective, the aim of this feasibility study is to apply the speckle technique for the assessment of the skin barrier in the Reconstructed Human Epidermis (RHE).
Materials And Methods: Speckle analysis as well as Raman microspectroscopy were performed on RHE samples at two maturation days, D17 and D20.
Results: Between D17 and D20, our study showed an increase in various Raman parameters, including stratum corneum percentage, lateral lipid packing, lipid-to-protein ratio, and protein secondary structure. Furthermore, the degree of light polarization and the speckle grain size also increased over this period.
Conclusion: The speckle technique proved to be effective for evaluating the skin barrier in Reconstructed Human Epidermis (RHE) models. Comparison with Raman validates this approach and provides comprehensive molecular and functional characterization of reconstructive skin models.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11024505 | PMC |
| http://dx.doi.org/10.1111/srt.13708 | DOI Listing |
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