AI Article Synopsis
- A thorough understanding of molecular and cellular processes during peri-implantation is crucial for improving pregnancy outcomes, which is when most pregnancy failures happen.
- Researchers analyzed single-cell transcriptomes of bovine embryos at different peri-implantation stages (days 12, 14, 16, and 18) to identify cell types and gene expression patterns in important embryonic structures.
- The study unveiled a new primitive trophoblast lineage and highlighted similarities and differences in developmental processes between bovines and other mammals, emphasizing the importance of cell communication for healthy early development.
Article Abstract
Supporting healthy pregnancy outcomes requires a comprehensive understanding of the molecular and cellular programs of peri-implantation development, when most pregnancy failure occurs. Here, we present single-cell transcriptomes of bovine peri-implantation embryo development at day 12, 14, 16, and 18 post-fertilization. We defined the cellular composition and gene expression of embryonic disc, hypoblast, and trophoblast lineages in bovine peri-implantation embryos, and identified markers and pathway signaling that represent distinct stages of bovine peri-implantation lineages; the expression of selected markers was validated in peri-implantation embryos. Using detailed time-course transcriptomic analyses, we revealed a previously unrecognized primitive trophoblast cell lineage. We also characterized conserved and divergence peri-implantation lineage programs between bovine and other mammalian species. Finally, we established cell-cell communication signaling underlies embryonic and extraembryonic cell interaction to ensure proper early development. These data provide foundational information to discover essential biological signaling underpinning bovine peri-implantation development.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11022056 | PMC |
| http://dx.doi.org/10.1016/j.isci.2024.109605 | DOI Listing |
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