AI Article Synopsis
- - The study focuses on translation initiation and scanning processes, examining how the proteins eIF4G1 and eIF1 affect the formation of the translation initiation complex (48S).
- - The researchers used a technique called TCP-seq to analyze the organization of 48S and discovered that AUG context plays a significant role in determining the efficiency of translation initiation, especially in non-leaky genes.
- - Their findings reveal distinct classes of initiation complexes, with one class being crucial for early initiation and another indicating a potential late initiation process, highlighting the dynamic nature of protein interactions during translation.
Article Abstract
Scanning and initiation are critical steps in translation. Here, we utilized translation complex profiling (TCP-seq) to investigate 48S organization and eIF4G1-eIF1 inhibition impact. We provide global views of scanning and leaky scanning, uncovering a central role of eIF4G1-eIF1 in their regulation. We confirm AUG context importance, with non-leaky genes featuring a Kozak context and cytosine at positions -1 and +5. Capturing 48S complexes associated with eIF1, eIF4G1, eIF3, and eIF2 through selective TCP-seq revealed that the eIF3-scanning ribosome is highly vulnerable to eIF4G1-eIF1 inhibition, and eIF1 tends to dissociate upon AUG recognition. Initiation-site footprint analysis revealed a class spanning -12 to +18/19 from the AUG, representing the entire 48S and enriched with eIF2, eIF1, and eIF4G1, indicative of early initiation. Another eIF3-dependent class extends up to +26 and exhibits reduced eIF2 and eIF4G1 association, suggesting a late/alternative initiation complex. Our analysis provides an overview of scanning, initiation, and evidence for conformational rearrangements in vivo.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.celrep.2024.114126 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Sputter-Coated TiO Films as Passivation and Hole Transfer Layers for Improved Energy Conversion with Solar Fuel WO/CuWO Photoanodes.
ACS Appl Mater Interfaces
December 2024
Department of Chemistry, University of California, Davis, One Shields Avenue, Davis, California 95616, United States.
Atomic-layer-deposited (ALD) "leaky" TiO has gained interest as a charge-selective protection layer for semiconductor solar fuel electrodes. Here, the use of sputter-deposited TiO layers as hole-selective contacts for WO/CuWO type-2 heterojunction water oxidation photoanodes is demonstrated for the first time. TiO protection layers with varying thicknesses (2 to 128 nm) were deposited by using the radio frequency (RF) magnetron sputtering technique.
View Article and Find Full Text PDFTripolar Bolalipids as Key Components of Sustained-Release Drug Delivery Systems.
Bioconjug Chem
December 2024
Univ. Bordeaux, CNRS, Bordeaux INP, CBMN, UMR 5248, F-33600 Pessac, France.
Controlling passive diffusion through an amphiphilic membrane is a key factor for the development of future smart generations of drug delivery systems. It also plays a crucial role in understanding fundamental biological systems through the design of new artificial cell models. We report herein a new concept of bolalipids designed as key components for the control of the membrane's permeability.
View Article and Find Full Text PDFTranslation Complex Profile Sequencing Allows Discrimination of Leaky Scanning and Reinitiation in Upstream Open Reading Frame-controlled Translation.
J Mol Biol
December 2024
School of Biochemistry and Cell Biology, University College Cork, Cork T12 K8AF, Ireland. Electronic address:
Upstream open reading frames (uORFs) are a class of translated regions (translons) in mRNA 5' leaders. uORFs are believed to be pervasive regulators of the translation of mammalian mRNAs. Some uORFs are highly repressive but others have little or no impact on downstream mRNA translation either due to inefficient recognition of their start codon(s) or/and due to efficient reinitiation after uORF translation.
View Article and Find Full Text PDFLike other volume electron microscopy approaches, automated tape-collecting ultramicrotomy (ATUM) enables imaging of serial sections deposited on thick plastic tapes by scanning electron microscopy (SEM). ATUM is unique in enabling hierarchical imaging and thus efficient screening for target structures, as needed for correlative light and electron microscopy. However, SEM of sections on tape can only access the section surface, thereby limiting the axial resolution to the typical size of cellular vesicles with an order of magnitude lower than the acquired xy resolution.
View Article and Find Full Text PDFLeaky ribosomal scanning enables tunable translation of bicistronic ORFs in green algae.
bioRxiv
July 2024
Department of Plant and Microbial Biology, University of California Berkeley, University of California, Berkeley, CA 94720, USA.
Advances in sequencing technology have unveiled examples of nucleus-encoded polycistronic genes, once considered rare. Exclusively polycistronic transcripts are prevalent in green algae, although the mechanism by which multiple polypeptides are translated from a single transcript is unknown. Here, we used bioinformatic and in vivo mutational analyses to evaluate competing mechanistic models for polycistronic expression in green algae.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!