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A Novel Hydrogel Sponge for Three-Dimensional Cell Culture.
Pharmaceutics
October 2024
Department of Pharmacy (DIFAR), University of Genoa, 16148 Genoa, Italy.
Background/objectives: Three-dimensional (3D) cell culture technologies allow us to overcome the constraints of two-dimensional methods in different fields like biochemistry and cell biology and in pharmaceutical in vitro tests. In this study, a novel 3D hydrogel sponge scaffold, composed of a crosslinked polyacrylic acid forming a porous matrix, has been developed and characterized.
Methods: The scaffold was obtained via an innovative procedure involving thermal treatment followed by a salt-leaching step on a matrix-containing polymer along with a gas-forming agent.
3D bioartificial stretchable scaffolds mimicking the mechanical hallmarks of human cardiac fibrotic tissue.
Int J Bioprint
May 2024
Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Turin, Turin, Italy.
Article Synopsis
- Human cardiac fibrotic tissues are stiffer than healthy tissues and can't contract on their own, highlighting the need for scaffolds that mimic their mechanical properties.
- This study developed bioartificial scaffolds made from PCL and gelatin methacryloyl (GelMA) that replicate the stiffness and stretchability of these fibrotic tissues for better integration with cardiac cells.
- Testing showed that scaffolds support human cardiac fibroblast culture, improve cell viability and organization, and facilitate the presence of smooth muscle actin-positive cells when subjected to dynamic conditions.
Protective Effect of Sulfur-Containing Heterocyclic Analogs Against Acrylamide-Induced Behavioral and Biochemical Alterations in Zebrafish.
Mol Neurobiol
August 2024
Toxicology and Pharmacology Laboratory, Department of Biotechnology, Faculty of Science and Humanities, SRM Institute of Science and Technology, Kattankulathur Chengalpattu District, 603203, Tamil Nadu, India.
Acrylamide (ACR) is a water-soluble monomer with broad consumer applications, even in foods due to thermal processes. Acute exposure to ACR may lead to neurotoxic effects such as ataxia and skeletal muscle weakness in humans and experimental animals. Oxidative stress is the primary pathway in ACR toxicity; therefore, this study aimed to evaluate the possible protective effect of benzo[b]thiophene analogs as an antioxidant drug for ACR poisoning.
View Article and Find Full Text PDFDevelopment of LC-MS/MS method for quantification of Lurasidone using volumetric absorptive microsampling (VAMS); a comparative study between dried blood and plasma samples.
J Chromatogr B Analyt Technol Biomed Life Sci
August 2024
SPPSPTM, SVKM's Narsee Monjee Institute of Management Studies, V.L. Mehta Road, Vile Parle (W), Mumbai 400056, India.
Article Synopsis
- - The development of alternative sampling techniques, like microsampling (<50 µL), aims to reduce the ecological impact of traditional bioanalytical methods that use toxic reagents and create biohazardous waste.
- - An LC-MS/MS method for Volumetric Absorptive Microsampling (VAMS) was successfully created to quantify Lurasidone, showing good precision and recovery rates, and proving effective even when samples are stored at room temperature.
- - The comparison of microsampling to conventional plasma techniques demonstrated that microsampling can provide comparable results while promoting better animal welfare and environmental protection, in line with the 3Rs principle.
A double ovulation protocol for Xenopus laevis produces doubled fertilisation yield and moderately transiently elevated corticosterone levels without loss of egg quality.
PLoS One
July 2024
Centre for Craniofacial Regeneration and Biology, King's College London, London, United Kingdom.
The African claw-toed frog, Xenopus laevis, is a well-established laboratory model for the biology of vertebrate oogenesis, fertilisation, and development at embryonic, larval, and metamorphic stages. For ovulation, X. laevis females are usually injected with chorionic gonadotropin, whereupon they lay typically hundreds to thousands of eggs in a day.
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