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Cultivation, cryopreservation, and transcriptomic studies of host-adapted and using enteroids. | LitMetric
and are major causes of severe diarrhea. Comparative studies of them are hampered by the lack of effective cultivation and cryopreservation methods, especially for . Here, we describe adapted murine enteroids for the cultivation and complete development of host-adapted and subtypes, producing oocysts infectious to mice. Using the system, we developed a cryopreservation method for isolates. In comparative RNA-seq analyses of cultures, the enteroid system generated significantly more host and pathogen responses than the conventional HCT-8 cell system. In particular, the infection was shown to upregulate PI3K-Akt, Ras, TNF, NF-κB, IL-17, MAPK, and innate immunity signaling pathways and downregulate host cell metabolism, and had significantly higher expression of parasite genes involved in oocyst formation. Therefore, the enteroid system provides a valuable tool for comparative studies of the biology of divergent species and isolates.
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