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Integration of multiomics analyses reveals unique insights into CD24-mediated immunosuppressive tumor microenvironment of breast cancer. | LitMetric

AI Article Synopsis

  • Tumor immunotherapy shows promise for breast cancer treatment, but challenges like low response rates and limited targets hinder its effectiveness; CD24 emerges as a key player in immune regulation.
  • * This study utilized advanced techniques like singleR and Monocle2 to analyze cell populations and communication, ultimately constructing a prognostic model based on 15 CD24-related genes from RNA sequencing data.
  • * Results indicate that patients with high CD24 expression have poorer survival rates due to an immunosuppressive environment, suggesting that targeting CD24 could enhance treatment outcomes in breast cancer.

Article Abstract

Background: Tumor immunotherapy brings new light and vitality to breast cancer patients, but low response rate and limitations of therapeutic targets become major obstacles to its clinical application. Recent studies have shown that CD24 is involved in an important process of tumor immune regulation in breast cancer and is a promising target for immunotherapy.

Methods: In this study, singleR was used to annotate each cell subpopulation after t-distributed stochastic neighbor embedding (t-SNE) methods. Pseudo-time trace analysis and cell communication were analyzed by Monocle2 package and CellChat, respectively. A prognostic model based on CD24-related genes was constructed using several machine learning methods. Multiple quantitative immunofluorescence (MQIF) was used to evaluate the spatial relationship between CD24PANCKcells and exhausted CD8T cells.

Results: Based on the scRNA-seq analysis, 1488 CD24-related differential genes were identified, and a risk model consisting of 15 prognostic characteristic genes was constructed by combining the bulk RNA-seq data. Patients were divided into high- and low-risk groups based on the median risk score. Immune landscape analysis showed that the low-risk group showed higher infiltration of immune-promoting cells and stronger immune reactivity. The results of cell communication demonstrated a strong interaction between CD24epithelial cells and CD8T cells. Subsequent MQIF demonstrated a strong interaction between CD24PANCK and exhausted CD8T cells with FOXP3 in breast cancer. Additionally, CD24PANCK and CD8FOXP3T cells were positively associated with lower survival rates.

Conclusion: This study highlights the importance of CD24breast cancer cells in clinical prognosis and immunosuppressive microenvironment, which may provide a new direction for improving patient outcomes.

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Source
http://dx.doi.org/10.1007/s00011-024-01882-9DOI Listing

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