Evaluation of , and 0.2% Chlorhexidine gluconate on disinfection of maxillofacial silicone material surface contaminated with microorganisms - An invitro study.

Unlabelled: Maintenance of the quality and hygiene of maxillofacial prosthesis allows to maintain the health of the residual tissues. Sampling of the maxillofacial prostheses has relieved presence of microbial colonization on silicone surfaces. Cleaning procedures of maxillofacial silicones are done using mechanical means or using adjunctive with chemical means. Cleaning with a 2-4% chlorhexidine gluconate spray or dipping in solution for a minute and then washing under running water can sufficiently condition to reduce the amount of bacterial contamination. Due to rising microorganism resistance and fewer adverse effects, phytoextracts appear to be a viable option. Additionally, the use of excipients derived from plants is provides new opportunities for the pharmaceutical industry into the creation of innovative pharmaceutical products that are sustainable.

Aim: To evaluate and compare the leaf extracts of and 0.2% chlorhexidine gluconate (CHX) on disinfection of maxillofacial silicone material surface contaminated with (

Methods: Of the 150 maxillofacial silicone elastomer silicone samples, 75 samples were contaminated with and 75 with . The contaminated disc was rolled on blood agar and pre-disinfection Colony Forming Units (CFU) were evaluated followed by subjecting the discs to disinfection protocols. The contaminated discs with and were disinfected using leaf extracts, leaf extracts and 0.2% CHX for 10 min. Post-disinfection CFUs were evaluated by rolling the disc on blood agar. The results were tabulated and analysed using dependent -test, one-way ANOVA and Tukeys multiple posthoc procedure.

Results: Pair-wise comparison of pre-and post-disinfection log CFU counts of gave a statistical significance between 0.2% CHX and leaf extract. No statistically significant results were found between 0.2% CHX and . Pair wise comparison of the log CFU from pre-disinfection to post-disinfection of gave a statistical significance between all the three groups.

Conclusions: In the present study leaf extract leaf extract have shown significant reduction in CFU of both the organisms. 0.2% CHX showed the most CFU reduction post disinfection of maxillofacial silicone material surface contaminated followed by leaf extracts and leaf extracts Given the limitations of the current research, leaf extract leaf extract can be used as an alternative for disinfection of maxillofacial silicone prosthesis.