AI Article Synopsis
- Scientists have a tough time figuring out how certain genes in malaria parasites work because many of them are not well understood.
- *They created a new method using something called CRISPR to test different genes and see how they help the parasites survive under drug treatment and stress.
- *Their method is easy to use and reliable, making it a great tool for studying important genes that were hard to look at before.
Article Abstract
It is a significant challenge to assess the functions of many uncharacterized genes in human malaria parasites. Here, we present a genetic screening tool to assess the contribution of essential genes from by the conditional CRISPR-/deadCas9-based interference and activation (i/a) systems. We screened both CRISPRi and CRISPRa sets, consisting of nine parasite lines per set targeting nine genes via their respective gRNAs. By conducting amplicon sequencing of gRNA loci, we identified the contribution of each targeted gene to parasite fitness upon drug (artemisinin, chloroquine) and stress (starvation, heat shock) treatment. The screening was highly reproducible, and the screening libraries were easily generated by transfection of mixed plasmids expressing different gRNAs. We demonstrated that this screening is straightforward, robust, and can provide a fast and efficient tool to study essential genes that have long presented a bottleneck in assessing their functions using existing genetic tools.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11015506 | PMC |
| http://dx.doi.org/10.1016/j.isci.2024.109602 | DOI Listing |
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