in mediating the proliferation of human ovarian cancer cells.

Background: Chloride channel-3 (), a crucial component of the voltage-gated chloride channel family, is implicated in numerous physiological and pathophysiological processes. This study aimed to investigate the characteristics of in pancancer and its influence on the immune response through the use of a range of databases. Concurrently, we assessed the impact of on the proliferation of ovarian cancer (OC) cells and explored its potential mechanisms.

Methods: We employed the Tumor Immune Estimation Resource (TIMER) 2.0 and Clinical Proteomic Tumor Analysis Consortium (CPTAC) databases to examine the messenger RNA (mRNA) and the protein expression of across various cancers. The prognostic significance of was evaluated using the Gene Expression Profiling Interactive Analysis 2.0 (GEPIA 2.0) database. The University of Alabama at Birmingham Cancer Data Analysis Portal (UALCAN) facilitated the analysis of promoter methylation levels. The association between expression and tumor-infiltrating immune cells was investigated using various algorithms. The cBioportal database facilitated the analysis of mutations and mutation sites across various cancers. The Tumor-Immune System Interactions Database (TISIDB) database was employed to explore the correlation between expression and immune or molecular subtypes across a variety of cancer types. We collected ovarian tissue samples, encompassing both normal ovarian and OC tissues. The human OC cell lines, SKOV3 cells and OVCAR433 cells, were cultured. expression was determined via reverse-transcription quantitative polymerase chain reaction (RT-qPCR), while phosphatidylinositol 3-kinase/Akt kinase (/) expression was detected using Western blot. We utilized small interfering RNA (siRNA) technology to suppress CLCN3 expression. The proliferative capacity of SKOV3 and OVCAR433 cells was assessed using the Cell Counting Kit 8 (CCK-8) assay.

Results: demonstrated an aberrant expression in a number of cancer types and was markedly reduced in OC tissues. Poor prognosis in cervical squamous cell cancer and myeloid leukemia was linked to excessive expression of . The examination of immune cell infiltration, which included CD8 T cells, B cells, T regulatory cells, and cancer-associated fibroblast cells, showed a strong association with aberrant expression. Following the use of siRNA technology, the ability of the ovarian carcinoma cell line SKOV3 and OVCAR433 to proliferate as well as the expression of / both increased.

Conclusions: is a possible biomarker for immune-related processes and the prognosis of cancer, and the / signaling pathway may affect OC cells' ability to proliferate.