AI Article Synopsis
- The study examined how snake venom components MT-II and MT-III enhance the phagocytic activity of macrophages, which are crucial immune cells.
- When treated with these venom components, macrophages showed improved ability to engulf non-opsonized particles, suggesting a boost in their immune response.
- However, this enhanced phagocytosis was significantly reduced when cells were pretreated with various inhibitors, highlighting the importance of the NO/sGC/GMP/PKG signaling pathway in this process.
Article Abstract
The phagocytic activity of macrophages activated with MT-II, a Lys-49 PLA homolog, and MT-III, an Asp-49 PLA, from Bothrops asper snake venom, was investigated in this study using a pharmacological approach. Stimulating thioglycollate-elicited macrophages with both venom components enhanced their ability to phagocytose non-opsonized zymosan particles. MT-II and MT-III-induced phagocytosis was drastically inhibited by pretreating cells with L-NAME, aminoguanidine or L-NIL, cNOS or iNOS inhibitors, or with ODQ (sGC inhibitor) or Rp-cGMPS (PKG inhibitor). These results indicate that the NO/sGC/GMP/PKG pathway plays an essential role in the β-glucan-mediated phagocytosis induced in macrophages by these venom-secretory PLAs.
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| http://dx.doi.org/10.1016/j.toxicon.2024.107716 | DOI Listing |
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