There has been an increasing demand for simultaneous detection of multiple analytes in one sample. Microbead-based platforms have been developed for multiplexed assays. However, most of the microbeads are made of non-biodegradable synthetic polymers, leading to environmental and human health concerns. In this study, we developed an environmentally friendly dextran microbeads as a new type of multi-analyte assay platform. Biodegradable dextran was utilized as the primary material. Highly uniform magnetic dextran microspheres were successfully synthesized using the Shirasu porous glass (SPG) membrane emulsification technique. To enhance the amount of surface functional groups for ligand conjugation, we coated the dextran microbeads with a layer of dendrimers via a simple electrostatic adsorption process. Subsequently, a unique and efficient click chemistry coupling technique was developed for the fluorescence encoding of the microspheres, enabling multiplexed detection. The dextran microbeads were tested for 3-plex cytokine analysis, and exhibited excellent biocompatibility, stable coding signals, low background noise and high sensitivity.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jcis.2024.04.061 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Polysaccharide-based drug delivery targeted approach for colon cancer treatment: A comprehensive review.
Int J Biol Macromol
January 2025
Department of Gastroenterology, Shengjing Hospital of China Medical University, Shenyang, Liaoning, China. Electronic address:
Colon cancer is a leading cause of cancer-related morbidity and mortality worldwide, necessitating advancements in therapeutic strategies to improve outcomes. Current treatment modalities, including surgery, chemotherapy, and radiation, are limited by systemic toxicity, low drug utilization rates, and off-target effects. Colon-targeted drug delivery systems (CDDS) offer a promising alternative by leveraging the colon's unique physiology, such as near-neutral pH and extended transit time, to achieve localized and controlled drug release.
View Article and Find Full Text PDFModeling vascular dynamics at the initial stage of endochondral ossification on a microfluidic chip using a human embryonic-stem-cell-derived organoid.
Regen Ther
March 2025
Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, Tokyo 113-8654, Japan.
Vascular interactions play a crucial role in embryogenesis, including skeletal development. During endochondral ossification, vascular networks are formed as mesenchymal cells condense and later invade skeletal elements to form the bone marrow. We and other groups developed a model of endochondral ossification by implanting human embryonic stem cell (hESC)-derived sclerotome into immunodeficient mice.
View Article and Find Full Text PDFReversible Ca signaling and enhanced paracellular transport in endothelial monolayer induced by acoustic bubbles and targeted microbeads.
Ultrason Sonochem
January 2025
Institute of Biomedical Engineering, Shenzhen Bay Laboratory, Shenzhen, China. Electronic address:
Article Synopsis
- Ultrasound combined with microbubble technology effectively opens the blood-brain barrier, allowing targeted drug delivery, but the underlying mechanisms, particularly regarding calcium signaling, need further exploration.
- Research showed that microbubbles create strong calcium responses and cell poration, while integrin-targeted microbeads lead to temporary calcium changes without damaging the cell membrane, highlighting different bioeffects in brain endothelial cells.
- The study also found that both microbubbles and microbeads enhance the permeability of endothelial cells for larger molecules, indicating potential improvements in drug delivery methods through better understanding of calcium signaling and cell junction dynamics.
Sodium Butyrate-Loaded Microspheres With Enhanced Bioavailability for Targeted Treatment of Intestinal Barrier Injury.
Adv Healthc Mater
December 2024
School of Materials and Chemistry, University of Shanghai for Science and Technology, No. 516 Jungong Road, Shanghai, 200093, China.
Intestinal barrier dysfunction is related to diseases such as inflammatory bowel disease (IBD) and severe acute pancreatitis (SAP). Butyrate and its derivatives (e.g.
View Article and Find Full Text PDFFabrication of stable hydrogel microspheres with hydrophobic shell using water-in-water (W/W) Pickering emulsion template.
Food Res Int
December 2024
Protein Research and Development Center, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, National Engineering Laboratory of Wheat & Corn Further Processing, South China University of Technology, Guangzhou 510640, PR China.
Article Synopsis
- - Hydrogel microspheres are gaining attention for food applications, but traditional methods, like water-in-oil emulsions, often require harmful surfactants and solvents, leading to swelling issues.
- - This study introduces a new approach using water-in-water emulsions with gelatin-in-dextran, eliminating toxic substances and minimizing energy use, and stabilizing the microspheres using zein particles modified with alginate.
- - The resulting core-shell microspheres are uniformly sized (18.8 μm) and stable under various conditions, with their sizes easily adjustable, making them promising for future food-related applications.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!