L. is an important medicinal plant, and flavonoids are among its main secondary metabolites. The R2R3-MYB transcription factor plays an irreplaceable role in plant growth, development, and secondary metabolism. In our study, we identified 159 R2R3-MYBs and analyzed their physical and chemical properties in . The protein length of 159 s varied from 107 to 1050 amino acids. The molecular weight ranged from 12.21 to 116.44 kDa. The isoelectric point was between 4.57 and 10.34. We constructed a phylogenetic tree of and R2R3-MYB family members, and members were divided into 33 subgroups. Transcriptome data analysis showed that the expression patterns of s in root, leaf, and flower were significantly different. Following the MeJA treatment of seedlings, five candidate PqMYB genes demonstrated a response. A correlation analysis of s and candidate flavonoid pathway genes showed that , , and had correlation coefficients that were higher than 0.8 with , , and , respectively. Furthermore, a transient expression assay confirmed that the three were localized in the nucleus. We speculated that these three s were related to flavonoid biosynthesis in . These results provided a theoretical basis and a new perspective for further understanding the R2R3-MYB gene family and the biosynthesis mechanism of secondary metabolites in .
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| http://dx.doi.org/10.3390/ijms25073709 | DOI Listing |
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