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Supplementation of Extender with Melatonin Improves the Motility, Mitochondrial Membrane Potential, and Fertilization Ability of Cryopreserved Brown-Marbled Grouper Sperm. | LitMetric

Supplementation of Extender with Melatonin Improves the Motility, Mitochondrial Membrane Potential, and Fertilization Ability of Cryopreserved Brown-Marbled Grouper Sperm.

Animals (Basel)

State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory of Aquatic Economic Animals, China-ASEAN Belt and Road Joint Laboratory on Mariculture Technology, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China.

Published: March 2024

AI Article Synopsis

  • Sperm cryopreservation is important for fish breeding and conservation, but oxidative damage can reduce sperm quality, making melatonin (MT) a useful antioxidant additive during the freezing process.
  • The study tested different MT concentrations (0, 0.1, 0.25, and 0.5 mg/mL) and found that 0.25 mg/mL improved sperm motility, mitochondrial health, and fertilization rates, while higher doses could be toxic.
  • Results suggest that incorporating optimal MT levels in sperm cryopreservation protocols could enhance the viability of brown-marbled grouper sperm for breeding and genetic improvement efforts.

Article Abstract

Sperm cryopreservation is a valuable tool for breeding, conservation, and genetic improvement in aquatic resources, while oxidative damage will cause a decline in sperm quality during this progress. Melatonin (MT), a natural antioxidant hormone, is used as an additive in sperm cryopreservation to reduce cellular damage from oxidative stress. Here, we aimed to investigate the effect of adding MT to the freezing medium in sperm cryopreservation of brown-marbled grouper (). Different concentrations of MT (0, 0.1, 0.25, and 0.5 mg/mL) were tested. We evaluated sperm motility, viability, apoptosis, mitochondrial membrane potential (MMP), and fertilization ability to assess the effects of MT supplementation. Our results demonstrated that the addition of MT to the extender improved the post-thaw motility, MMP, and fertilization ability of brown-marbled grouper sperm. The total motility, curvilinear velocity, straight linear velocity, and average path velocity in MT-treated groups (0.1 and 0.25 mg/mL) exhibited significantly higher values than that of the control group. A higher MMP ( < 0.05) was observed in the group treated with 0.25 mg/mL MT, suggesting that supplementation of MT in the extender might be able to protect mitochondrial membrane integrity effectively. Regarding fertilizing ability, 0.25 mg/mL MT yielded a significantly higher hatching rate than the control. An adverse effect was found with the concentration of MT up to 0.5 mg/mL, suggesting the possible toxicity of a high-dose addition. In this study, we optimized the sperm cryopreservation protocol of brown-marbled grouper, which might be valuable for sperm cryopreservation and sample commercialization of groupers and other fish.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11010917PMC
http://dx.doi.org/10.3390/ani14070995DOI Listing

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