A new structurally simple fluorescent probe based on chromone was designed and synthesized, and its structure was fully characterized using various analytical techniques. The probe displays a high selectivity and sensitivity for sensing Fe with a "turn-off" fluorescence response over other metal ions in a DMSO/HO (4:1, /) solution. The experiment results show that the probe is stable over a wide pH range of 2.0-12.0. The detection limit for Fe was calculated to be 0.044 μmol•L. The molar ratio method indicated that the binding mode between the probe and Fe is a 1:1 complex formation. HR-MS and density functional theory (DFT) calculations were also performed to further confirm the recognition mechanism. Both fluorescence imaging experiments and the MTT assay demonstrated that the probe was suitable for detecting intracellular Fe and no significant cytotoxicity in living cells.
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| http://dx.doi.org/10.3390/molecules29071504 | DOI Listing |
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