The Identification and Analysis of the Self-Incompatibility Pollen Determinant Factor in .

Self-incompatibility is a widespread genetic mechanism found in flowering plants. It plays a crucial role in preventing inbreeding and promoting outcrossing. The genes that control self-incompatibility in plants are typically determined by the S-locus female determinant factor and the S-locus male determinant factor. In the Solanaceae family, the male determinant factor is often the gene. In this research, we cloned and analyzed 13 genes from the genome, which are located on chromosome 2 and close to the physical location of the S-locus female determinant factor S-RNase, covering a region of approximately 90.4 Mb. The amino acid sequence identity of the 13 is 58.46%, and they all possess relatively conserved motifs and typical domains, without introns. A co-linearity analysis revealed that there are no tandemly repeated genes in the genes, and that there are two pairs of co-linear genes between and the tomato, which also belongs to the Solanaceae family. A phylogenetic analysis indicates that the members can be divided into six groups, and it was found that the 13 are clustered with the genes of tobacco and to varying degrees, potentially serving as pollen determinant factors regulating self-incompatibility in . The results for the gene expression patterns suggest that is only expressed in pollen tissue. The results of the yeast two-hybrid assay showed that the C-terminal region of lacking the domain can interact with S-RNase. This study provides theoretical data for further investigation into the functions of members, particularly for the identification of pollen determinant factors regulating self-incompatibility in .