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The protective effect of zinc oxide nanoparticles on boar sperm during preservation at 17 °C.
Anim Reprod
January 2025
Hebei Key Laboratory of Animal Diversity, College of Life Sciences, Langfang Normal University, Hebei Langfang, China.
More than 90% of spermatozoa of boars in pork producing countries is stored in liquid at 17 °C; however, the quality of these spermatozoa is affected by bacterial breeding and oxidative damage. This study analyzed sperm quality and sperm capacitation after storage to study the effects of the effects of ZnO nanoparticles (ZnO NPs) supplementation on seminal plasma (SP)-free sperm preservation. We investigated the effects of adding 20, 50, 100 and 200 μg/mL of ZnO NPs to a seminal free boar sperm diluent over a 7-day period at 17 °C to assess the changes in non-capacitated/capacitated sperm quality parameters, antioxidant capacity, ATP content and extent of protein tyrosine phosphorylation.
View Article and Find Full Text PDFFungal lignocellulolytic enzymes: an in silico and full factorial design approach.
World J Microbiol Biotechnol
January 2025
Graduate Program in Bioscience Technologies, Universidade Tecnológica Federal do Paraná, Toledo, Paraná, Brazil.
Efficient degradation of lignocellulosic biomass is key for the production of value-added products, contributing to sustainable and renewable solutions. This study employs a two-step approach to evaluate lignocellulolytic enzymes of Ceratocystis paradoxa, Colletotrichum falcatum, and Sporisorium scitamineum. First, an in silico genomic analysis was conducted to predict the potential enzyme groups produced by these fungi.
View Article and Find Full Text PDFPathogenesis-Associated Bacterial Amyloids: The Network of Interactions.
Biochemistry (Mosc)
December 2024
Faculty of Biology, St. Petersburg State University, St. Petersburg, 199034, Russia. ARRAY(0x5ae2b7af6df8).
Amyloids are protein fibrils with a characteristic cross-β structure that is responsible for the unusual resistance of amyloids to various physical and chemical factors, as well as numerous pathogenic and functional consequences of amyloidogenesis. The greatest diversity of functional amyloids was identified in bacteria. The majority of bacterial amyloids are involved in virulence and pathogenesis either via facilitating formation of biofilms and adaptation of bacteria to colonization of a host organism or through direct regulation of toxicity.
View Article and Find Full Text PDF(P)ppGpp synthetase Rel facilitates cellulose formation of biofilm by regulating glycosyltransferase in Brucella abortus.
Int J Biol Macromol
January 2025
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China; Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University; Yangling, Shaanxi 712100, China. Electronic address:
Biofilms are complex adhesive structures that establish chronic infection and allow robust protection from external stressors such as antibiotics. Cellulose as one of the compositions of bacteria biofilm which protect bacteria from stress, host immune responses and resistance to antibiotics. Bacterial stress responses are regulated via guanosine pentaphosphate and tetraphosphate (p)ppGpp.
View Article and Find Full Text PDFTwo-in-one strategy to enhance the stability of TiCT in transition metal ion solutions.
J Colloid Interface Sci
January 2025
College of Materials Science and Engineering, Hunan University, Changsha 410082 PR China. Electronic address:
Although MXenes have attracted significant attention across diverse fields, they exhibit a pronounced susceptibility to oxidation in aqueous environments, with oxidation significantly accelerated in the presence of transition metal ions (TMI) such as Fe and Cu. This limitation impedes the synthesis of transition metal compounds/MXene-based composites and their potential for functional applications. In this study, we elucidate the mechanism of accelerated oxidation of TiCT is that Fe promotes the electron loss in TiCT, thus leading to an increased production of hydroxyl radicals (OH) to oxidize TiCT.
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