AI Article Synopsis
- - The pro-protein convertase FURIN (PCSK3) plays a key role in various biological processes related to diseases like infections, cancer, and cardiovascular conditions.
- - Researchers conducted a study using CRISPR technology to create different knockout clones of FURIN in U937 monocytic cells to explore how inhibiting FURIN impacts myeloid cells.
- - The results revealed distinct changes in immune functions like phagocytosis, inflammation, and gene expression in FURIN-deficient cells, shedding light on FURIN's potential involvement in cardiovascular diseases.
Article Abstract
The pro-protein convertase FURIN (PCSK3) is implicated in a wide range of normal and pathological biological processes such as infectious diseases, cancer and cardiovascular diseases. Previously, we performed a systemic inhibition of FURIN in a mouse model of atherosclerosis and demonstrated significant plaque reduction and alterations in macrophage function. To understand the cellular mechanisms affected by FURIN inhibition in myeloid cells, we optimized a CRISPR-mediated gene deletion protocol for successfully deriving hemizygous (HZ) and nullizygous (NZ) knockout clones in U937 monocytic cells using lipotransfection-based procedures and a dual guide RNA delivery strategy. We observed differences in monocyte and macrophage functions involving phagocytosis, lipid accumulation, cell migration, inflammatory gene expression, cytokine release patterns, secreted proteomics (cytokines) and whole-genome transcriptomics between wild-type, HZ and NZ FURIN clones. These studies provide a mechanistic basis on the possible roles of myeloid cell FURIN in cardiovascular disorders.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11154428 | PMC |
| http://dx.doi.org/10.3390/cells13070588 | DOI Listing |
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