Genotyping of spp. in wild rats leads to first time detection of serovar Mozdok in Serbia.

Introduction: This study aimed to investigate the prevalence and molecular characterization of species in Belgrade, Serbia, an area where this disease is underexplored. Specifically, the study sought to employ molecular and multilocus sequence typing analyses to fill the gap in understanding the diversity and distribution of species within the region.

Methods: A comprehensive molecular analysis was conducted on kidney samples obtained from Norway rats () in the urban environment. The study utilized molecular diagnostic techniques including real-time PCR targeting the gene and performing sequence-based typing schemes utilizing and genes. These methodologies were applied to ascertain the presence and characterize different species and serovars, respectively.

Results: The findings revealed the presence of two species and three separate serovars in the Belgrade area. This study identified the presence of serovar Mozdok in Serbia for the first time, a significant discovery previously undocumented in the region. This pioneering investigation sheds light on the molecular diversity and prevalence of species in Serbia.

Discussion: The study underscores the importance of employing molecular typing methods to gain insights into the epidemiology and characterization of species. These findings significantly contribute to both local and global perspectives on leptospirosis epidemiology, providing vital insights for the development of effective control strategies and interventions.

Summary: In our recent study, we explored the presence and performed molecular typing of the species, the bacteria responsible for leptospirosis, in wild rats in Serbia. This was the first time such a study was conducted in the region. Leptospirosis is a serious disease that affects both animals and humans, often transmitted through contact with water contaminated by infected animals. Our focus was on understanding which types of were present in these animals. Excitingly, we discovered a particular strain of , known as serovar Mozdok, for the first time in Serbia. This finding is significant because it sheds light on the presence and spread of different serovars in Serbia. It also raises awareness about the potential health risks associated with this serovar, which was previously unknown in the area. Our work fits into a broader context of disease surveillance and public health. By identifying the types of present in a specific region, we can better understand the risks to public health and take steps to prevent and control the spread of leptospirosis. This discovery is not just important for scientists studying infectious diseases; it has real implications for public health officials, veterinarians, and anyone concerned with preventing and treating leptospirosis. Our findings highlight the need for ongoing monitoring of in wildlife and synanthropic fauna, to protect both animal and human health.