Effect of dietary supplementation extract on growth performance and muscle nutrition of by gut bacterial mediation.

Nowadays, is a typical cultured fish in south China due to its resource decline in the Pearl River. Meanwhile, since antibiotics had been banned internationally, Chinese medical herbal plant serving as alternative to antibiotics has been adopted in aquaculture. In the present study, to ensure the health growth of . , extract of traditional medical herbal plant was dietary supplemented and a series experiments were performed, including growth performance determination, physiological/biochemical detection, nutrition analysis, histology analysis, and 16S rRNA amplicon sequencing. Growth performance enhancement was determined since the weight gain rate (WGR), specific growth rate (SGR), and condition factor (CF) of . increased as feeding inclusion . extract. Interestingly, the total content of muscle fatty acids ascended via supplementing . extract at middle level, in which group the activities of superoxide dismutase (SOD) and catalase (CAT) significantly increased and thus retarded the lipid peroxidation process (manifesting as malondialdehyde (MDA) content rising). Additionally, immune response and inflammatory reaction was stimulated in low and high level . extract added groups, indicating a suitable dosage of . extract benefited in safety production. Moreover, gut microbiota community varied hugely as daily supplementation . extract and the keystone species were tightly related to lipid transformation, which ultimately led to fatty acids composition variation. Our results confirmed that dietary supplementation . extract at the middle level (0.5‰, w/w) is suitable for serving as feed additive in healthful aquaculture of . .