Infectious hematopoietic necrosis (IHN), caused by IHN virus, is a highly contagious and lethal disease that seriously hampers the development of rainbow trout (Oncorhynchus mykiss) aquaculture. However, the immune response mechanism of rainbow trout underlying IHNV infection remains largely unknown. MicroRNAs act as post-transcriptional regulators of gene expression and perform a crucial role in fish immune response. Herein, the regulatory mechanism and function of miR-206 in rainbow trout resistance to IHNV were investigated by overexpression and silencing. The expression analysis showed that miR-206 and its potential target receptor-interacting serine/threonine-protein kinase 2 (RIP2) exhibited significant time-dependent changes in headkidney, spleen and rainbow trout primary liver cells infected with IHNV and their expression displayed a negative correlation. In vitro, the interaction between miR-206 and RIP2 was verified by luciferase reporter assay, and miR-206 silencing in rainbow trout primary liver cells markedly increased RIP2 and interferon (IFN) expression but significantly decreased IHNV copies, and opposite results were obtained after miR-206 overexpression or RIP2 knockdown. In vivo, overexpressed miR-206 with agomiR resulted in a decrease in the expression of RIP2 and IFN in liver, headkidney and spleen. This study revealed the key role of miR-206 in anti-IHNV, which provided potential for anti-viral drug screening in rainbow trout.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.fsi.2024.109552 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Transient cognitive impacts of oxygen deprivation caused by catch-and-release angling.
Biol Lett
January 2025
Centre for Ecology and Evolution in Microbial Model Systems (EEMiS), Department of Biology and Environmental Science, Faculty of Health and Life Sciences, Linnaeus University, Kalmar 39231, Sweden.
Vertebrate brain function is particularly sensitive to the effects of hypoxia, with even brief periods of oxygen deprivation causing significant brain damage and impaired cognitive abilities. This study is the first to investigate the cognitive consequences of hypoxia in fish, specifically induced by exhaustive exercise and air exposure, conditions commonly encountered during catch-and-release (C&R) practices in recreational fishing. Angling exerts substantial pressure on inland fish populations, underscoring the need for sustainable practices like C&R.
View Article and Find Full Text PDFBrodifacoum causes coagulopathy, hemorrhages, and mortality in rainbow trout (Oncorhynchus mykiss) at environmentally relevant hepatic residue concentrations.
Ecotoxicol Environ Saf
January 2025
Section IV 1.2 Biocides, German Environment Agency, Dessau-Roßlau 06813, Germany.
Widely used second-generation anticoagulant rodenticides like brodifacoum are classified as persistent, bioaccumulative, and toxic. Widespread exposure of terrestrial and avian non-target species is well-known and recently hepatic anticoagulant rodenticide residues have been detected in wild fish. However, no sufficient data exist to interpret the effects of these findings on fish health.
View Article and Find Full Text PDFEffects of Feed Supplementation With Fulvic Acid on the Systemic and Mucosal Protective Mechanisms of Juvenile Rainbow Trout (Oncorhynchus mykiss).
J Anim Physiol Anim Nutr (Berl)
January 2025
Faculty of Fisheries and Protection of Waters, South Bohemian Research Centre of Aquaculture and Biodiversity of Hydrocenoses, Institute of Aquaculture and Protection of Waters, University of South Bohemia, České Budějovice, Czech Republic.
Rainbow trout (Oncorhynchus mykiss) is an important fish species raised in aquaculture, but it is susceptible to stress, infections diseases. The present study aimed to determine the effects of fulvic acid feed addition on the systemic and mucosal protective mechanisms of juvenile rainbow trout and to elucidate the underlying molecular mechanisms of changes in the gut. Rainbow trout (4.
View Article and Find Full Text PDFProlonging Shelf Life and Meat Quality of Rainbow Trout () by Immersing in Pine Nut () Extract During Cold Storage.
Food Sci Nutr
January 2025
Caspian Sea Ecology Research Center Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization Mazandaran Iran.
Rainbow trout () is a freshwater fish susceptible to chemical and microbial spoilage, limiting its shelf life. This study aimed to enhance and extend the rainbow trout fillets' shelf life stored at 4°C ± 1°C through an immersion treatment using ultrasound-assisted, defatted pine nut ( Wallich) extracts at concentrations of 1% and 2% (w/v), compared to the control group (0% pine nut). Evaluations were conducted at storage intervals of 0, 4, 8, 12, 16, and 20 days.
View Article and Find Full Text PDFN-methyladenosine RNA methylation regulates microplastics-induced cell senescence in the rainbow trout liver.
Sci Total Environ
January 2025
School of Bioengineering and Technology, Tianshui Normal University, Gansu Province, PR China. Electronic address:
Microplastics are prevalent in aquatic ecosystems, impacting various forms of aquatic life, including fish. In this study, Rainbow trout (Oncorhynchus mykiss) were exposed to two concentrations of microplastics (0 and 500 μg/L) over a 14-day period, during which a comprehensive analysis was conducted to assess the liver accumulation of microplastics and their effects on oxidative stress, the liver response, and transcriptomics. Our findings indicated that microplastics significantly accumulated in the liver and activated the antioxidant system in fish by enhancing the activity of antioxidant enzymes.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!