AI Article Synopsis
- The study introduces a method called STSS that enhances nanopore signals by controlling the length of ssDNA produced through rolling circle amplification using AcyNTP.
- This technique effectively converts short oligonucleotide targets into longer ssDNAs, which generate stronger translocation currents in nanopore measurements.
- By labeling the RCA products with tetrahedral structures and isothermal amplicons, the method improves resolution, signal specificity, and expands the range of detectable targets.
Article Abstract
Through controlling the ssDNA product length of rolling circle amplification with AcyNTP, here we develop a nanopore signal enhancement strategy (STSS), which can successfully transfer the short oligonucleotide targets into long ssDNAs with appropriate lengths that can generate significant translocation currents. By labelling the RCA product with tags such as tetrahedral structures and isothermal amplicons, the resolution, signal specificity, and target range of the STSS can be further extended.
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| http://dx.doi.org/10.1039/d4cc00961d | DOI Listing |
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