The Effect of Short-Term NAD3® Supplementation on Circulating Adult Stem Cells in Healthy Individuals Aged 40-70 Years.

Objective This study aimed to assess the impact of acute and short-term supplementation with NAD3, a theacrine-containing supplement, on circulating adult stem cell numbers in a healthy male and female population aged 40-70 years. Methods This was a double-blind, placebo-controlled crossover study with 12 participants randomized to receive either NAD3® or a placebo for seven days. Blood samples were collected after an overnight fast, before and after the seven-day supplementation period, and one and two hours after the final supplement dose. Using flow cytometry, circulating stem cells, including lymphocytoid CD34 stem cells (CD45CD34), stem cells associated with vascular maintenance and repair (CD45CD34CD309), CD34 stem cells linked to a progenitor phenotype (CD45CD34CD309), circulating endothelial stem cells (CD45CD31CD309), and mesenchymal stem cells (CD45CD90) were quantified. Results Acute NAD3 supplementation did not result in the mobilization of stem cells from the bone marrow. However, seven days of daily NAD3 supplementation resulted in selective changes in circulating stem cell numbers. A significant time*treatment interaction was observed for CD45CD34cells (p=0.04) and CD45CD34CD309cells (p=0.04), indicating a decrease in cell numbers with supplementation. There was also a trend toward an increase in circulating endothelial cells (p=0.08) with seven days of NAD3supplementation. Conclusion Short-term NAD3® supplementation demonstrated an effect on the quantity of bone marrow-derived stem cells in circulation. The study suggests that this theacrine-containing supplement may play a role in modulating adult stem cell populations, emphasizing the potential impact of NAD3® on regenerative processes. Further research with extended supplementation periods and larger sample sizes is warranted to elucidate the functional consequences of these changes and explore the therapeutic implications for age-related declines in stem cell function.