AI Article Synopsis
- Wolbachia pipientis is a symbiotic bacterium that affects various physiological aspects of its host arthropods, including mosquitoes like Aedes fluviatilis.
- The study investigates how the enzyme GSK3β influences immune changes in this relationship, finding that its inhibition leads to increased glycogen levels and a higher Wolbachia population.
- The research concludes that GSK3β plays a crucial role in managing the immune response in Ae. fluviatilis, specifically in controlling the population of Wolbachia within the host.
Article Abstract
Wolbachia pipientis is a maternally transmitted symbiotic bacterium that mainly colonizes arthropods, potentially affecting different aspects of the host's physiology, e.g., reproduction, immunity, and metabolism. It has been shown that Wolbachia modulates glycogen metabolism in mosquito Aedes fluviatilis (Ae. fluviatilis). Glycogen synthesis is controlled by the enzyme GSK3, which is also involved in immune responses in both vertebrate and invertebrate organisms. Here we investigated the mechanisms behind immune changes mediated by glycogen synthase kinase β (GSK3β) in the symbiosis between Ae. fluviatilis and W. pipientis using a GSK3β inhibitor or RNAi-mediated gene silencing. GSK3β inhibition or knockdown increased glycogen content and Wolbachia population, together with a reduction in Relish2 and gambicin transcripts. Furthermore, knockdown of Relish2 or Caspar revealed that the immunodeficiency pathway acts to control Wolbachia numbers in the host. In conclusion, we describe for the first time the involvement of GSK3β in Ae. fluviatilis immune response, acting to control the Wolbachia endosymbiotic population.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11154636 | PMC |
| http://dx.doi.org/10.1016/j.jbc.2024.107272 | DOI Listing |
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Article Synopsis
- Wolbachia pipientis is a symbiotic bacterium that affects various physiological aspects of its host arthropods, including mosquitoes like Aedes fluviatilis.
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Wolbachia pipientis is a maternally transmitted bacterium that mostly colonizes arthropods, including the mosquito Aedes fluviatilis, potentially affecting different aspects of host physiology. This intracellular bacterium prefers gonadal tissue cells, interfering with the reproductive cycle of insects, arachnids, crustaceans, and nematodes. Wolbachia's ability to modulate the host's reproduction is related to its success in prevalence and frequency.
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