AI Article Synopsis
- Osteosarcoma is the most prevalent primary bone cancer in young individuals, but advancements in treatment have not significantly improved patient outcomes.
- The patient-derived xenograft (PDX) model effectively preserves the unique characteristics of human tumors, making it a valuable tool for cancer research.
- This article outlines a detailed protocol for creating a PDX mouse model using human osteosarcoma tissue, which helps in studying tumor behavior, drug resistance, and developing personalized treatment strategies.
Article Abstract
Osteosarcoma is the most common primary malignant bone tumor in children and adolescents. Despite the development of new treatment plans in recent years, the prognosis for osteosarcoma patients has not significantly improved. Therefore, it is crucial to establish a robust preclinical model with high fidelity. The patient-derived xenograft (PDX) model faithfully preserves the genetic, epigenetic, and heterogeneous characteristics of human malignancies for each patient. Consequently, PDX models are considered authentic in vivo models for studying various cancers in transformation studies. This article presents a comprehensive protocol for creating and maintaining a PDX mouse model that accurately mirrors the morphological features of human osteosarcoma. This involves the immediate transplantation of freshly resected human osteosarcoma tissue into immunocompromised mice, followed by successive passaging. The described model serves as a platform for studying the growth, drug resistance, relapse, and metastasis of osteosarcoma. Additionally, it aids in screening the target therapeutics and establishing personalized treatment schemes.
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| http://dx.doi.org/10.3791/66491 | DOI Listing |
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