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| http://dx.doi.org/10.3389/fcvm.2024.1392073 | DOI Listing |
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Transcriptomic analysis of the inhibition mechanisms against by antibacterial aptamer B4.
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State Key Laboratory of Mariculture Breeding, Engineering Research Centre of the Modern Technology for Eel Industry, Ministry of Education, Fisheries College of Jimei University, Xiamen, China.
is a common bacterial pathogen in aquaculture, often leading to visceral white spot disease in large yellow croakers (). Previous studies have found that certain aptamers show an efficient antibacterial effect against this pathogen. In this study, we analyzed the transcriptome of to get insights into the antibacterial and inhibitions mechanisms following exposure to the aptamer B4.
View Article and Find Full Text PDFThe future of cell-free synthetic biology.
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Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.
Cell-free synthetic biology aims at the targeted replication, design, and modification of life processes in open systems by breaking free of constraints such as cell membrane barriers and living cell growth. The beginnings of this systematized technology, which took place in the last century, were used to explore the secrets of life. Currently, with its easy integration with other technologies or disciplines, cell-free synthetic biology is developing into a powerful and effective means of understanding, exploiting, and extending the structure and function of natural living systems.
View Article and Find Full Text PDFEnzymatic synthesis of reactive RNA probes containing squaramate-linked cytidine or adenosine for bioconjugations and cross-linking with lysine-containing peptides and proteins.
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Protein-RNA interactions play important biological roles and hence reactive RNA probes for cross-linking with proteins are important tools in their identification and study. To this end, we designed and synthesized 5'-O-triphosphates bearing a reactive squaramate group attached to position 5 of cytidine or position 7 of 7-deazaadenosine and used them as substrates for polymerase synthesis of modified RNA. In vitro transcription with T7 RNA polymerase or primer extension using TGK polymerase was used for synthesis of squaramate-modified RNA probes which underwent covalent bioconjugations with amine-linked fluorophore and lysine-containing peptides and proteins including several viral RNA polymerases or HIV reverse transcriptase.
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Cell types are traditionally thought to be specified and stabilized by gene regulatory networks. Here, we explore how chromatin memory contributes to the specification and stabilization of cell states. Through pervasive, local, feedback loops, chromatin memory enables cell states that were initially unstable to become stable.
View Article and Find Full Text PDFIn vitro and ex vivo screening of microRNA combinations with enhanced cell penetrating peptides to stimulate intervertebral disc regeneration.
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- Low back pain is mainly caused by degeneration of the intervertebral disc, prompting research into microRNA (miRNA) therapies which can modulate discogenic factors and inhibit degeneration.
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