AI Article Synopsis
- - The pro-B cell stage is crucial for commitment to the B cell lineage, and the transcription factor YY1 plays a key role in this process; knocking out YY1 during this stage prevents cells from committing to B cells.
- - Instead of developing into B cells, YY1 knockout pro-B cells can differentiate into T lineage cells in specific experimental conditions, demonstrating their ability to switch lineages.
- - Single-cell RNA sequencing reveals that these YY1 knockout pro-B cells display a variety of transcript profiles, indicating a significant level of lineage plasticity and suggesting that YY1 is important for lineage commitment across different cell types.
Article Abstract
During B cell development, cells progress through multiple developmental stages with the pro-B cell stage defining commitment to the B cell lineage. YY1 is a ubiquitous transcription factor that is capable of both activation and repression functions. We find here that knockout of YY1 at the pro-B cell stage eliminates B lineage commitment. YY1 knockout pro-B cells can generate T lineage cells using the OP9- DL4 feeder system, as well as after injection into sub-lethally irradiated Rag1 mice. These T lineage-like cells lose their B lineage transcript profile and gain a T cell lineage profile. Single cell-RNA-seq experiments showed that as YY1 knockout pro-B cells transition into T lineage cells, various cell clusters adopt transcript profiles representing a multiplicity of hematopoietic lineages indicating unusual lineage plasticity. Given the ubiquitous nature of YY1 and its dual activation and repression functions, YY1 likely regulates commitment in multiple cell lineages.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10996465 | PMC |
| http://dx.doi.org/10.1101/2024.03.22.586298 | DOI Listing |
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