CRISPR/Cas9 methods are a powerful approach to edit the genome of . To convert existing lines to driver lines in a secondary school classroom setting, we applied the CRISPR-based genetic approach to a collection of 'driver' lines. The integration of the coat color marker into homology-assisted CRISPR knock-in (HACK) enabled visual selection of -to- conversions using brightfield stereo-microscopy available in a broader set of standard classrooms. Here, we report the successful conversion of eleven lines with expression in neuropeptide-expressing cells into corresponding, novel drivers. The conversion was confirmed by - and -specific GFP reporter gene expression. This curriculum was successfully implemented in a summer course running 16 hours/week for seven weeks. The modularity, flexibility, and compactness of this course should enable development of similar classes in secondary schools and undergraduate curricula, to provide opportunities for experience-based science instruction, and university-secondary school collaborations that simultaneously fulfill research needs in the community of science.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10996655PMC
http://dx.doi.org/10.1101/2024.03.28.587232DOI Listing

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