Long Non-Coding RNA NUTM2A-AS1/miR-376a-3p/PRMT5 Axis Promotes Prostate Cancer Progression.

Background: In recent years, significant attention has been directed towards long non-coding RNA NUT family member 2A antisense RNA 1 () for its oncogenic role in tumours. This study aimed to investigate the functional and molecular mechanisms underlying in prostate cancer (PCa).

Methods: , , and protein arginine methyltransferase 5 () levels were assessed in PCa samples and matched non-cancerous prostate samples. The DU145 cell line was conditioned to undergo transfection with relevant plasmids, and a cell counting kit-8 assay was performed to evaluate cell proliferation. A Transwell assay was conducted to analyse cell migration or invasion. Cell apoptosis was assessed using an annexin V-fluorescein isothiocyanate/propidium iodide apoptosis detection kit and flow cytometry. A tumour sphere formation assay was conducted to assess the ability of PCa cells to form tumour spheres.

Results: We found elevated expression of and and decreased expression of in PCa samples. Inhibition of or overexpression of led to reduced cell proliferation and diminished cancer stem cell-like traits . regulated through competitive absorption, thereby modulating . Up-regulation of nullified the therapeutic effects of inhibiting or overexpressing in DU145 cells.

Conclusions: promotes cancer stem cell-like traits in PCa cells by targeting through . Therefore, these -based novel insights into tumour therapy hold promise for patients with PCa.