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Development and Validation of LAMP Assays for Distinguishing MPXV Clades with Fluorescent and Colorimetric Readouts.
Biosensors (Basel)
January 2025
School of Biomedical Sciences and Engineering, Koç University, 34450 Istanbul, Turkey.
Human monkeypox (Mpox) is a zoonotic disease caused by the Monkeypox virus (MPXV). As of 14 August 2024, the World Health Organization (WHO) has declared it a global health emergency. For Mpox, this was the second public health emergency of global significance in the past two years.
View Article and Find Full Text PDFThe nuclear condensates of ESE3/EHF induce cellular senescence without the associated inflammatory secretory phenotype in pancreatic ductal adenocarcinoma.
Cancer Lett
December 2024
Pancreas Center, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, State Key Laboratory of Druggability Evaluation and Systematic Translational Medicine, Tianjin Key Laboratory of Digestive Cancer, Tianjin's Clinical Research Center for Cancer, Tianjin, PR China. Electronic address:
Senescent cells are in a stable state of cell cycle arrest, leading to a natural barrier to tumorigenesis. Senescent cells secrete a pool of molecules, including cytokines, chemokines, proteases, and growth factors, termed the senescence-associated secretory phenotype (SASP), paradoxically contributing to pro-tumorigenic processes. However, the mechanism for regulating senescence and SASP in tumor cells remains unclear.
View Article and Find Full Text PDFTetrahedral DNA-Based Ternary Recognition Ratiometric Fluorescent Probes for Real-Time In Situ Resolving Lysosome Subpopulations in Living Cells via Cl, Ca, and pH.
Anal Chem
October 2024
Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, P.O. Box 332, Shenyang 110819, China.
Article Synopsis
- Lysosomes are essential organelles that help maintain cellular balance, and they can be categorized into different groups based on their ion levels of chloride (Cl), calcium (Ca), and pH.
- Researchers have created a new fluorescence probe using tetrahedral DNA nanostructures to simultaneously measure Cl, Ca, and pH in lysosomes, which was previously difficult.
- The study found significant differences in lysosomal subpopulations in Niemann-Pick disease model cells compared to control cells, suggesting the probe could aid in understanding lysosomal behavior and treating related diseases.
FixNCut: A Practical Guide to Sample Preservation by Reversible Fixation for Single Cell Assays.
Bio Protoc
September 2024
Adelaide Centre for Epigenetics (ACE), University of Adelaide, Adelaide, South Australia, Australia.
The quality of standard single-cell experiments often depends on the immediate processing of cells or tissues post-harvest to preserve fragile and vulnerable cell populations, unless the samples are adequately fixed and stored. Despite the recent rise in popularity of probe-based and aldehyde-fixed RNA assays, these methods face limitations in species and target availability and are not suitable for immunoprofiling or assessing chromatin accessibility. Recently, a reversible fixation strategy known as FixNCut has been successfully deployed to separate sampling from downstream applications in a reproducible and robust manner, avoiding stress or necrosis-related artifacts.
View Article and Find Full Text PDFA novel BODIPY-based fluorescent probe for naked-eye detection of the highly alkaline pH.
Spectrochim Acta A Mol Biomol Spectrosc
January 2025
School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address:
A novel alkaline pH-responsive probe based on an asymmetric aza-BODIPY was synthesized in a one-pot Schiff base formation reaction. This pH-sensitive probe comprises an asymmetric aza-BODIPY as the luminescent core, with a benzothiazole moiety connected via an imine bond serving as the recognition site. The probe exhibits a turn-off fluorescence response upon exposure to alkaline pH (9.
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