AI Article Synopsis
- Phycoerythrin (PE) is a valuable phycobiliprotein useful as a food colorant and potential medicine, extracted from Porphyridium purpureum using deep eutectic solvent (DES) and ultrasound-assisted extraction (UAE).
- The study explored 31 DESs for cell wall disruption and determined that five glycerol-based DESs significantly boosted B-PE yields, achieving up to 13.5 times more than water.
- The extraction process was deemed environmentally friendly, and the final purified B-PE achieved high purity levels comparable to commercial products after rigorous purification steps.
Article Abstract
Phycoerythrin (PE) is a phycobiliprotein holding great potential as a high-value food colorant and medicine. Deep eutectic solvent (DES)-based ultrasound-assisted extraction (UAE) was applied to extract B-PE by disrupting the resistant polysaccharide cell wall of Porphyridium purpureum. The solubility of cell wall monomers in 31 DESs was predicted using COSMO-RS. Five glycerol-based DESs were tested for extraction, all of which showed significantly higher B-PE yields by up to 13.5 folds than water. The DES-dependent B-PE extraction efficiencies were proposedly associated with different cell disrupting capabilities and protein stabilizing effects of DESs. The DES-based UAE method could be considered green according to a metric assessment tool, AGREEprep. The crude extract containing DES was further subjected to aqueous two-phase system, two-step ammonium sulfate precipitation, and ultrafiltration processes. The final purified B-PE had a PE purity ratio of 3.60 and a PC purity ratio of 0.08, comparable to the purity of commercial products.
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| http://dx.doi.org/10.1016/j.foodchem.2024.139196 | DOI Listing |
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