AI Article Synopsis
- The study aimed to see if pre-treating Wharton's Jelly Mesenchymal Stem Cells (WJ-MSCs) with valproic acid (VPA) would enhance their ability to migrate and improve kidney recovery after ischemia-reperfusion injury (IRI) in rats.
- Researchers conducted experiments with 150 rats divided into five groups and assessed recovery through various analyses over different time intervals.
- Results showed that WJ-MSCs and VPA improved kidney function and antioxidant levels more effectively than IRI alone, with the combination treatment providing the best outcomes by promoting specific gene expressions linked to kidney protection.
Article Abstract
Objective: To determine whether WJ-MSCs pretreated with VPA would enhance their migration to improve functional recovery of renal IRI in rats.
Methods: 150 Sprague-Dawley rats were distributed into 5 groups; Sham, IRI, WJ-MSC, VPA, and WJ-MSCs + VPA. 10 rats were sacrificed after 3, 5, and 7 days. Role of WJ-MSCs pretreated with VPA was evaluated by assessment of renal function, antioxidant enzymes together with renal histopathological and immunohistopathological analyses and finally by molecular studies.
Results: WJ-MSCs and VPA significantly improved renal function and increased antioxidants compared to IRI group. Regarding gene expression, WJ-MSCs and VPA decreased BAX and TGF-β1, up-regulated Akt, PI3K, BCL2, SDF1α, and CXCR4 related to IRI. Additionally, WJ-MSCs pretreated with VPA improved the measured parameters more than either treatment alone.
Conclusion: WJ-MSCs isolated from the umbilical cord and pretreated with VPA defended the kidney against IRI by more easily homing to the site of injury.
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| http://dx.doi.org/10.1016/j.abb.2024.109985 | DOI Listing |
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