Vasorelaxant and hypotensive effects of an ethanolic extract of and its main compound quercetin 3-methyl ether 3'-O-β-xylopyranoside.

plants were traditionally used to treat diseases associated with endothelial dysfunction. The present study investigated the effects of an ethanolic extract of Willd. (commonly named water lily, WL) and its main compound (quercetin 3-methyl ether 3'-O--xylopyranoside) on vascular function in rats. The vasorelaxant effects of the WL extract and its main compound and their underlying mechanisms of action were evaluated on isolated mesenteric arteries from Wistar rats. Blood pressure and heart rate were measured in anesthetized rats after infusion (i.v) of vehicle, WL extract, and compound (at 0.01, 0.025, 0.05, 0.1, 0.5, and 1 mg/kg). Nifedipine was used as a positive control. Both WL extract and compound induced vasorelaxant effects (with EC of 0.08 ± 0.01 mg/mL and 42.8 ± 6.3 µM, respectively) that were reduced by endothelium removal. A significant decrease in these relaxations was observed with L-NAME but not with apamin-charybdotoxin or indomethacin. In the endothelium-denuded condition, WL extract-induced relaxation was enhanced by 4-aminopyridine and glibenclamide, while iberiotoxin and ODQ (1H-[1,2,4]oxadiazolo[4,3-a]quinoxaline-1-one) had no effect. In contrast, compound -induced relaxation was not changed by any of these inhibitors. Both WL extract and compound enhanced sodium nitroprusside-induced relaxation and inhibited receptor-operated Ca channels. Only the WL extract was able to reduce PE-induced contraction ( < 0.001). As compared to the vehicle, the infusion of WL extract and compound lowered systolic and diastolic blood pressure. Interestingly, the hypotensive effect of the compound was similar to that of nifedipine. The rebound tachycardia found at the highest dose of nifedipine was not observed with the WL extract or compound ( < 0.05). Our study demonstrated a vasorelaxant effect of the WL extract and its main compound quercetin 3-methyl ether 3'-O--xylopyranoside, relying on the potentiation of the NO-cGMP pathway and calcium inhibitory effects. These vasorelaxant effects were associated with a potent hypotensive effect, providing pharmacological evidence for the traditional use of this plant.