Enhanced capillary delivery with nanobubble-mediated blood-brain barrier opening and advanced high resolution vascular segmentation.

J Control Release

Department of Biomedical Engineering, Tel Aviv University, Tel Aviv, Israel; The Sagol School of Neuroscience, Tel Aviv University, Tel Aviv, Israel. Electronic address:

Published: May 2024

AI Article Synopsis

  • * A dual-dye labeling technique along with advanced microscopy and deep learning was employed to accurately assess BBB opening at a detailed level while saving time in image analysis.
  • * Results showed that nanobubbles significantly improve BBB opening in smaller capillaries compared to traditional microbubbles, while maintaining similar effectiveness in larger blood vessels, highlighting their potential as superior agents for BBB modulation.

Article Abstract

Overcoming the blood-brain barrier (BBB) is essential to enhance brain therapy. Here, we utilized nanobubbles with focused ultrasound for targeted and improved BBB opening in mice. A microscopy technique method assessed BBB opening at a single blood vessel resolution employing a dual-dye labeling technique using green fluorescent molecules to label blood vessels and Evans blue brain-impermeable dye for quantifying BBB extravasation. A deep learning architecture enabled blood vessels segmentation, delivering comparable accuracy to manual segmentation with a significant time reduction. Segmentation outcomes were applied to the Evans blue channel to quantify extravasation of each blood vessel. Results were compared to microbubble-mediated BBB opening, where reduced extravasation was observed in capillaries with a diameter of 2-6 μm. In comparison, nanobubbles yield an improved opening in these capillaries, and equivalent efficacy to that of microbubbles in larger vessels. These results indicate the potential of nanobubbles to serve as enhanced agents for BBB opening, amplifying bioeffects in capillaries while preserving comparable opening in larger vessels.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jconrel.2024.04.001DOI Listing

Publication Analysis

Top Keywords

bbb opening
16
blood-brain barrier
8
blood vessel
8
blood vessels
8
evans blue
8
larger vessels
8
opening
7
bbb
6
enhanced capillary
4
capillary delivery
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!