AI Article Synopsis
- Understanding bacterial movement from human feces may help diagnose and treat inflammatory bowel disease.
- The text outlines a method for conducting a microbial swarming assay to isolate and identify these bacteria using techniques like MALDI-TOF mass spectrometry and whole-genome sequencing.
- It emphasizes the importance of validating results by repeating the swarming assay to confirm the observed behavior of the bacteria.
Article Abstract
Understanding the specific movements of bacteria isolated from human feces can serve as a novel diagnostic and therapeutic tool for inflammatory bowel disease. Here, we present a protocol for a microbial swarming assay and to isolate the bacteria responsible for swarming activity. We describe steps for identifying bacteria using MALDI-TOF mass spectrometry and whole-genome sequencing. We then detail procedures for validating findings by observing the same swarming phenotype upon reperforming the swarming assay. For complete details on the use and execution of this protocol, please refer to De et al..
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10999858 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2024.102961 | DOI Listing |
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