Surface plasmon resonance (SPR) is an optical effect at an electron-rich surface that enables affinity measurements of biomolecules in real time. It is label free and versatile, not limited to proteins, nucleic acids, and small molecules. SPR is a widely accepted method to measure not only affinity of molecular interactions but also association and dissociation rates of such interactions. In this chapter, we describe a general method to measure the affinity of a small molecule drug, MRTX849, to GDP bound HRAS, KRAS, and NRAS.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-0716-3822-4_8 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Green synthesis of silver nanoparticles from the endophytic fungus Panax notoginseng and their antioxidant and antimicrobial activities and effects on cherry tomato preservation.
Int J Food Microbiol
January 2025
College of Animal Science and Technology, Yangtze University, Jingzhou 434023, China. Electronic address:
This study investigated endophytic fungi isolated from the medicinal plant Panax notoginseng. Among these, the endophytic fungus SQ3, identified as Chaetomium globosum, was capable of reducing silver ions to form metallic silver nanoparticles. The green-synthesized silver nanoparticles (AgNPs) presented a distinct surface plasmon resonance peak at 424 nm, with particle sizes between 2.
View Article and Find Full Text PDFSodium butyrate regulates macrophage polarization by TGR5/β-arrestin2 in vitro.
Mol Med
January 2025
Department of Critical Care Medicine, Renmin Hospital of Wuhan University, 238 Jiefang Road, Wuchang, Wuhan, 430060, Hubei, China.
Background: Macrophages play an important role in the pathogenesis of ulcerative colitis (UC). We will explore the effects of sodium butyrate (SB) on macrophage function.
Methods: The targets of butyric acid were identified using SwissTargetPrediction database and surface plasmon resonance (SPR).
AI integration into wavelength-based SPR biosensing: Advancements in spectroscopic analysis and detection.
Anal Chim Acta
March 2025
Artificial Intelligence Research Center, Chang Gung University, Taoyuan, 333323, Taiwan; Department of Artificial Intelligence, College of Intelligent Computing, Chang Gung University, Taoyuan, 333323, Taiwan. Electronic address:
Background: In recent years, employing deep learning methods in the biosensing area has significantly reduced data analysis time and enhanced data interpretation and prediction accuracy. In some SPR fields, research teams have further enhanced detection capabilities using deep learning techniques. However, the application of deep learning to spectroscopic surface plasmon resonance (SPR) biosensors has not been reported.
View Article and Find Full Text PDFInteraction and cleavage of cell and plasma proteins by the platelet-aggregating serine protease PA-BJ of Bothrops jararaca venom.
Biochimie
January 2025
Laboratory of Applied Toxinology, Center of Toxins, Immune-Response and Cell Signaling (CeTICS), Butantan Institute, São Paulo, Brazil. Electronic address:
PA-BJ is a serine protease present in Bothrops jararaca venom that triggers platelet aggregation and granule secretion by activating the protease-activated receptors PAR-1 and PAR-4, without clotting fibrinogen. These receptors also have a relevant role in endothelial cells, however, the interaction of PA-BJ with other membrane-bound or soluble targets is not known. Here we explored the activity of PA-BJ on endothelial cell receptor, cytoskeleton, and coagulation proteins in vitro, and show the degradation of fibrinogen and protein C, and the limited proteolysis of actin, EPCR, PAR-1, and thrombomodulin.
View Article and Find Full Text PDFHyperoside mitigates PCOS-associated adipogenesis and insulin resistance by regulating NCOA2-mediated PPAR-γ ubiquitination and degradation.
Life Sci
January 2025
Departments of Gynaecology and Obstetrics, The Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai 200137, China. Electronic address:
Aims: Polycystic ovary syndrome (PCOS) is closely associated with metabolic disorders such as insulin resistance and obesity, but the role of adipogenesis in its pathophysiology remains unclear. This study investigates the role of adipogenesis in PCOS development and evaluates whether hyperoside (HPS), an anti-adipogenic herbal compound, can improve PCOS by inhibiting adipogenesis.
Main Methods: A combination of in vivo and in vitro models was used to assess the impact of HPS on ovarian function, insulin resistance, and adipogenesis.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!