Tobacco, a vital economic crop, had its quality post-curing significantly influenced by starch content. Nonetheless, the existing process parameters during curing were inadequate to satisfy the starch degradation requirements. Microorganisms exhibit inherent advantages in starch degradation, offering significant potential in the tobacco curing process. Our study concentrated on the microbial populations on the surface of tobacco leaves and in the rhizosphere soil. A strain capable of starch degradation, designated as BS3, was successfully isolated and identified as by phylogenetic tree analysis based on 16SrDNA sequence. The application of BS3 on tobacco significantly enhanced enzyme activity and accelerated starch degradation during the curing process. Furthermore, analyses of the metagenome, transcriptome, and metabolome indicated that the BS3 strain facilitated starch degradation by regulating surface microbiota composition and affecting genes related to starch hydrolyzed protein and key metabolites in tobacco leaves. This study offered new strategies for efficiently improving the quality of tobacco leaves.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10985783 | PMC |
| http://dx.doi.org/10.3389/fbioe.2024.1332113 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effects of multienzyme supplementation on energy and nutrient digestibility in various feed ingredients for pregnant gilts.
J Anim Sci
January 2025
Department of Animal Science, South Dakota State University, Brookings, SD, 57007, USA.
The utilization of exogenous fiber-degrading enzymes in commercial swine diets is a strategy to increase the nutrient and energy density of poorly digestible ingredients. In a prior set of studies, dietary multienzyme blend (MEblend) supplementation increased the apparent total tract digestibility (ATTD) of nutrients, non-starch polysaccharides, and energy in complete high-fibrous gestation diets by 6% when fed to gestating sows. The current study aimed to determine the effects of MEblend (containing xylanase, β-glucanase, cellulase, amylase, protease, pectinase, and invertase activities) supplementation on ATTD of energy and nutrients of individual feedstuffs commonly used in gestating sow diets across major pork-producing regions worldwide, which differ in their fibrous components.
View Article and Find Full Text PDFScale-Up of Tailor-Made Onsite Enzyme Blend From Cassava Peels for Industrial Bioethanol Production.
ScientificWorldJournal
January 2025
Department of Biochemistry and Biotechnology, College of Science, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Bioethanol production is one of the key alternatives for fossil fuel use due to climate change. The study seeks to upscale tailor-made onsite enzyme blends for the bioconversion of cassava peels to bioethanol in simultaneous saccharification and fermentation (SSF) process using cassava peels-degrading fungi. The starch and cellulose contents of peels were determined.
View Article and Find Full Text PDFGastrointestinal complications of hepatic glycogen storage disease: a national survey questionnaire study in China.
Orphanet J Rare Dis
January 2025
Department of Pediatrics, Guangdong Provincial People's Hospital, The Second School of Clinical Medicine, Guangdong Academy of Medical Sciences, Southern Medical University, Guangzhou, 510080, China.
Background: Hepatic glycogen storage diseases (GSD) are inborn errors of metabolism with abnormal storage or utilization of glycogen, a complex disease with significant genetic heterogeneity and similar clinical manifestations. This study aimed to describe the gastrointestinal symptoms and endoscopic features of hepatic GSD, including types Ia, Ib, III, VI, and IX, to provide evidence for etiology and treatment.
Methods: A national cohort survey questionnaire was distributed to patients diagnosed with GSD type Ia, Ib, III, VI, and IX through genetic testing or their parents in mainland China in May 2022.
Fermentative profile and bacterial community structure of whole-plant triticale silage () with or without the addition of and .
mSphere
January 2025
Animal nutrition and feed science, College of Animal Science and Technology, Shihezi University, Shihezi, China.
This study aimed to investigate the effects of and on the chemical composition, fermentation characteristics, bacterial communities, and predicted metabolic pathways of whole-plant triticale silage (). Fresh triticale harvested at the milk stage was ensiled in sterile distilled water (CON), (ST), (LP), and a combination of and (LS) for 3, 7, 15, and 30 days. During ensiling, the pH and water-soluble carbohydrate (WSC) content in the inoculated groups was significantly lower than those in the CON group ( < 0.
View Article and Find Full Text PDFPreparation and Adsorption Properties of Sodium Trimetaphosphate Crosslinked Porous Corn Starch.
Biopolymers
March 2025
Guangxi Key Laboratory of Natural Polymer Chemistry and Physics, College of Chemistry and Materials, Nanning Normal University, Nanning, People's Republic of China.
The crosslinked porous corn starch was prepared by two steps: the native corn starch was hydrolyzed by α-amylase and glucoamylase, then the porous corn was crosslinked by sodium trimetaphosphate (STMP). The morphology and size of granules, spherulites, crystal type, molecular structure, swelling properties, thermal stability and adsorption properties of the crosslinked porous starch were investigated. The results indicated that a lot of holes formed in the porous starch, and the particle size of starch granules decreased.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!