The present study evaluated the production potential of CH, carboxylic acids and alcohols from a mixed culture enriched using synthetic syngas. The influence of syngas concentration on the microbial community and products productivity and selectivity was investigated. The results demonstrated the enrichment of a mesophilic mixed culture capable of converting CO and H mainly to CH and acetate, along with butyrate. The selectivity values showed that acetate production was enhanced during the first cycle in all conditions tested (up to 20 %), while CH was the main product generated during following cycles. Concretely, CH selectivity remained unaffected by syngas concentration, reaching a stable value of 41.6 ± 2.0 %. On the other hand, butyrate selectivity was only representative at the highest syngas concentration and lower pH values (26.1 ± 5.8 %), where the H consumption was completely inhibited. Thus, pH was identified as a key parameter for both butyrate synthesis and the development of hydrogenotrophic activity.
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